壁虎醇提物有效成份分离与抗消化道肿瘤作用分析

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1、-------目录第4章结果................................................194.1对GCPs的分析....................................................................................................194.1.1高效液相色谱法（HPLC）对GCPs的分析结果........................................194.1.2聚丙烯酰氨凝胶电泳法（Trince

2、ne-SDS-PAGE）对GCPs的分析结果.....194.2体外抗肿瘤活性实验结果....................................................................................194.2.1细胞形态学观察.............................................................................................194.2.2GCPs对消化道肿瘤细胞增殖的抑制作用.........

3、..........................................194.2.3HepG2细胞凋亡的形态学改变.....................................................................224.2.4TUNEL法检测HepG2细胞的凋亡率..........................................................224.2.5细胞内凋亡蛋白bcl-2和bax的表达水平........................

4、...........................234.3体内抗肿瘤活性实验结果....................................................................................234.3.1GCPs对小鼠H22移植瘤的生长抑制作用...................................................234.3.2GCPs对荷瘤小鼠免疫功能影响..........................................

5、.........................244.3.3GCPs对瘤组织中VEGF表达的影响...........................................................244.3.4GCPs对H22移植瘤小鼠血清TNF-α，IL-6含量的影响...........................24第5章讨论................................................275.1GCPs的提取及成分分析........................

6、..............................................................275.1.1针对GCPs的提取方法.................................................................................275.1.2对GCPs的分析.............................................................................................

7、285.2GCPs对癌细胞增殖的抑制作用...........................................................................285.2.1GCPs抑制三种消化道癌细胞的增殖...........................................................285.2.2体内实验证实GCPs具有抑制H22移植瘤生长的作用.............................295.3GCPs具有抑制肿瘤增殖和诱导其凋亡的作用....

8、...............................................29 5.3.1GCPs抑制肝癌HepG2细胞中PCNA的表达.......................................