硼对大豆再生系统与遗传转化的影响初探

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1、-英文摘要硼对大豆再生系统及遗传转化的影响初探EffectofBoronontheRegenerationandTransformationofSoybean(Glycinemax)AbstractSoybean(Glycinemax)isanimportantcrops.However,therearemanyfactorswhichaffectitsyield,suchasthecropdiseases,insectpests,weedsanddroughtinpracticalsoybeanproduction.Withthedevelopmentofthegen

2、eticengineeringtechnology,thetransgenicapproacheshavebeentriedtoovercometheseproblems.Duetothelowtransformationandregenerationrate,afewofcasesoftransgenicsoybeansweresuccessful.Toestablisheffectivetransformationandregenerationsystemsarestillhotinthesoybeantransformation.Boronplaysanimpor

3、tantroleinmaintainingthestructuralandfunctionalintegrityofcellwallandcellularmembraneinplant.Inthepresentexperiment,theinfluenceofboricconcentrationinthemediumontheinductionofsoybeancallusandmultipleshootsaswellasthetransformationeffeciencyofagrobacterium-mediatedreportgene(GUS)wereinvas

4、tigatedbyculturingtheexplantfromseedlingsofJilinsmallseedNo.7soybean.ThetransientexpressionratesofGUSgeneexpressionwerecalculatedbasedonthehistochemicalmethodandthestabletransformedshootswerefurtherdetectedbyPCRandDotblotting,inordertoexploretheefficiencyofsoybeantransformationinasuitabl

5、eboricacidconcentration.Resultsshowedthatlowboronconcentrationhadnosignificanteffectonthestructureofcallusinashorttime,whilehighboronculturemedium(50~300mg/L)resultedinstructuresofcalluscellsloosen.Whentheboricacidishigherthan300mg/L,theexplantsandcouldnotformnormalcallusduetotheserioust

6、oxicity.Moreover,boricacidconcentrationwith5.0mg/Lto10mg/L,aroundthenormalconcentrationofboricacid(6.2mg/L),hadthehightestmultipleshootsinductionrates.Bothhighboronandlowboronmediumwouldinhibittheinductionofmultipleshootsofcotyledonarynode,especiallyathighlevelboronmultipleshootsinductio

7、nratesweresignificantlyreduced.Butthosemultipleshootswerestillhadtheregenerationability.II---硼对大豆再生系统及遗传转化的影响初探英文摘要However,agrobacteriummediatedtransformationefficiencyinsoybeanexplantweresignificantlyrisenat30mg/Lboricacidconcentrationcomparedwithnormalboricacidenvironme