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ID:27243557
大小:760.50 KB
页数:47页
时间:2018-12-02
《重度子痫前期子宫小动脉平滑肌细胞大电导钙激活钾通道的改变》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、-Singlechannelcurrentwasamplifiedandfilteredbypatchclampamplifier(CEZ-2200),thenimportedintothecomputer,pClamp6.0softwarewasusedtorecordthedigitaldataandClampfit10.1softwarewasusedtoanalyzedata.ComparethechangeofBKCacurrentsignalintheuterinearterysmoothmusclecellsbetweenthepregnantwomanInn
2、ormalpregnancyandseverepreeclampsia.Results:1.AcuteenzymeseparationofUterinearterialsmoothmusclecells:Thesingleuterinearterialsmoothmusclecellwhichwasdissociatedbyenzymesolutioncanmaintainitsphysiologicalfunctions,anditcanbeusedinpatchclampexperiments2.Thepropertiesofsinglechannelcurrentso
3、fBKCachannelsinUterinearterialsmoothmusclecellswereasfollows:(1)SinglechannelcurrentsofBKCawererecordedininside-outpatch.Thevalueofsinglechannelconductancewas207.2±12.7pS.(2)Thechannelshaddistinctvoltagedependentandcalciumdependentcharacteristics.(3)Inoutside-outpatch(Vm=+30mV),theactivati
4、onofBKCachannelcouldbeblockedby200nMIbTXcompletely.3.comperedthesinglechannelcurrentsofthenormotensivepregnantgroupBKCachannelwiththeseverepreeclampsiagroup:incell-attachedpatch(Vm=+40mV),theThemeanopenprobabilityoftheNormotensivepregnantgroupwas0.7±0.0017,Theamplitudeofvoltagewas7.627±0.7
5、27pA,themeanopentimewas7.216±1.003msms,themeanclosetimewas879.445±149.716ms,theThemeanopenprobabilityoftheSeverepreeclampsiagroupwas0.17±0.0005,Theamplitudeofvoltagewas4.399±0.464pA,themeanopen5----timewas1.642±0.348ms,themeanclosetimewas930.791±264.157ms.comperedwiththeNormotensivepregnan
6、tgroup,thethemeanopentimeandtheamplitudeofvoltageoftheSeverepreeclampsiagroupsignificantlydecreased,thetooftheSeverepreeclampsiagroupsignificantlydecreasedalso.themeanclosetimeoftheSeverepreeclampsiagroupNosignificantdecreased.Conclusion:1.Thisexperimentadoptsthetwo-stepacuteenzymeseparati
7、ontodissociatedpregnancyuterinearterioles,thenwegotthesmoothmusclecellswhichweresuitablefortheperforatedpatchclampexperiment.2.Large-conductanceCa2+-activatedpotassiumchannels(BKCa)arehighlyexpressedinthesmoothmusclecellswhichisolatedfromsmalluterusartery,theB
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