安胃汤对大鼠慢性萎缩性胃炎EGF和EGFR表达的PCR分析论文

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1、·目录中文摘要················································1英文摘要················································3引言·················································7正文·················································91.实验材料和方法·····························

2、·············91.1实验材料···············································91.2实验方法·············································〃〃〃〃·111.3统计学方法········································162.实验结果··················································162.1动物外观观察······

3、·····································162.2胃粘膜大体形态观察·····································162.3胃粘膜光学显微镜观察···································162.4血清中表皮生长因子及其受体含量检测结果············172.5实时荧光定量PCR结果···········································192.6电泳图片·········

4、············································193讨论·················································204.存在的问题和展望·······································32结论···············································34参考文献·············································

5、··35附录···············································39综述···············································40致谢···············································48攻读学位期间发表的学术论文目录···················49作者声明·············································50个人简历···

6、············································51····中文摘要目的:观察安胃汤对慢性萎缩性胃炎大鼠血中表皮生长因子及其受体表达量的影响，从而探讨安胃汤治疗慢性萎缩性胃炎疗效的分子机制，从基因水平上阐释名老中医治疗慢性萎缩性胃炎的学术理论。方法:健康清洁级雄性Wistar大鼠84只，体重140~170克，完全随机分为空白对照组（A组）、病理模型组（B组）、安胃汤大剂量组（C组）、安胃汤中剂量组（D组）、安胃汤小剂量组（E组）、维酶素对照组（F组）、胃复春

7、对照组（G组）共七组，每组12只。空白组给予正常饮食进水，其余六组采用甲基硝基亚硝基胍（MNNG）溶液建立慢性萎缩性胃炎大鼠模型。于造模成功后开始，安胃汤大剂量组、安胃汤中剂量组、安胃汤小剂量组、维酶素对照组、胃复春对照组分别给予药物灌胃治疗4周；空白组，病理模型组同时给予每天每只2ml生理盐水灌胃。各组大鼠禁食12ｈ，将大鼠麻醉，股动静脉取血，ACD抗凝，淋巴细胞分离液分离有核血细胞，提取有核细胞总RNA，经反转录成cDNA，设计合成EGF和EGFR特异性引物，通过real-timePCR的方法

8、检测7组大鼠血中EGF和EGFR基因表达的差异；同时剖腹取胃，沿胃大弯剪开，并向胃窦近幽门口至胃大弯方向取1.0㎝×1.0㎝×0.5㎝胃粘膜组织，臵于10%甲醛固定，作常规石腊包埋切片，做胃黏膜组织病理观察。结论:1.胃黏膜大体形态观察：通过观察皱襞多少、走向，黏膜色泽，是否有出血等情况，发现病理模型组皱襞减少或消失，走向紊乱，表面粘液少，黏膜色泽淡白或灰白，且部分伴有散在出血点。经安胃汤大、中、小剂量组，维酶素对照组、胃复春对照组治疗后，胃黏膜损害均有不同程度的改善，以安胃汤大剂