基于蛋白质组学筛选弓形虫病候选诊断抗原

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1、-------目录基于血清蛋白质组学筛选弓形虫病候选诊断抗原.................................3摘要..................................................................................................3Abstract.................................................................................................51前言.

2、................................................................................................82材料与方法.....................................................................................112.1实验材料..............................................................................

3、......112.1.1BALB/c小鼠.......................................................................112.1.2弓形虫...............................................................................112.1.3血清标本...........................................................................112.1.4主

4、要仪器...........................................................................112.1.5主要试剂..........................................................................122.1.6主要试剂配制....................................................................122.2实验方法......................

5、..............................................................172.2.1RH株弓形虫速殖子的收集...................................................172.2.2弓形虫可溶性总抗原（WSA）的二维电泳（2-DE）.............182.2.3诊断抗原的血清学筛选及质谱鉴定.......................................222.2.4逆转录聚合酶链反应（RT-PCR）.............

6、.............................262.2.5克隆载体的连接、转化和鉴定..............................................282.2.6表达载体的连接、鉴定及转化..............................................312.2.7重组蛋白ROP2186–533（rROP2186–533）的诱导、表达、鉴定..322.2.8rROP2186–533和WSA的应用—ELISA法................................33

7、2.2.9统计学方法...........................................................................343结果...............................................................................................353.1WSA的血清蛋白质组学分析.....................................................353.2.rROP2186–533的

8、克隆、表达及鉴定...........................................413.3rROP2186–533的应用....