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1、硒化壳聚糖通过下调PML作者：邓守恒，曹凤军，蔡晓军，许涛，邓守平，陈萍【摘要】目的研究硒化壳聚糖对急性早幼粒白血病细胞株NB4增殖和凋亡的影响，探讨这种影响与PML-RARα融合蛋白表达的关系。方法应用MTT法检测硒化壳聚糖对细胞增殖的影响，应用AO/EB荧光染色法共聚焦显微镜下观察诱导凋亡作用，应用L-RARα融合蛋白含量的变化。结果硒化壳聚糖可对体外培养的NB4细胞产生剂量和时间依赖性的抑制作用，50mg/L至100mg/L硒化壳聚糖作用NB4细胞24h可诱导细胞出现明显的凋亡现象，经硒化壳聚糖处理24h后，NB4细胞内PML-RARα融合蛋白含量明显减少。结
2、论硒化壳聚糖可通过下调PML-RARα融合蛋白含量对NB4细胞产生抑制增殖和诱导凋亡作用。【关键词】硒化壳聚糖;NB4细胞；增殖；凋亡；PML-RARα融合蛋白 Chitosan［β(1,4)-2-amino-2-deoxy-D-glucose］,analmostnontoxicbio-polysaccharideinnature,acologicaleffectsincludingantitumor,antimicrobialandantioxidantproperties［1,2］byreport,isoftenusedasdrugpreparationmate
3、rialsduetoitsexcellentbiopatibility［3］.Selenium,belongingtothesamefamilyintheperiodictableofelementsorelement(Arsenictrioxidehaslongbeenusedasananti-leukemiadruginclinicalpractice)hasbeenfoundtoincreaseapoptosisofAcutePromyelocyticLeukemia(APL)celllinesNB4cellthroughPI3K/Aktpathcannotb
4、eeasilyabsorbedbycancercells,havenarroalllethaldose,andsoon.Therefore,,i.e.Chitosanselenium［5］,viachitosanandseleniousacidinacidsolution,usingthemixedmetalionsasthecatalyst,,shouldalsohaveanti-leukemiaeffectandhavemuchsmallersideeffectsthaninorganicseleniumandarsenide［6］. Inthischitos
5、anonproliferationandapoptosisofhumanAPLcelllinesNB4cellinvitro．AndtheabilityofSeleniumchitosantomodulatePML-RARαfusionprotEinsthatmightcontributetoNB4cellsurvivalethods 1.1DrugsandantibodiesSeleniumchitosanentofpharmaceuticalChemistry，FujianmedicalUniversity；SeleniumcontentSantaCruzBi
6、otechnology．I-l640mediumsupplemented，streptomycin100mg/L，benzylpenicillin100kU/Lat37%inahumidified5%CO2．Afterincubationfor24h,exponentiallygrochitosanofdifferentconcentrations(50,l00,200mg/L)for24h～48h．MTTinetheproliferativeeffectsofdrugsontumorcellsandAO/EBfluoresentstainingmethodSele
7、niumchitosan-treatedcellsmol/L，pH8.0，NaCl150mmol/L，dithiothreitol1mmol/L,edeticacid0.5mmol/L，nonidetP400.1％，sodiumdodecylsulfate0.1％phenylmethylsulfonlyfluoride100mg/L)supplementedg/L，leupeptin2mg/L，andsodiumorthovanadate100μmol/L．Appropriateproteinamounts(20μg)dodecylsulfate-poly-ac
