csb-e09745h 人网膜素(omentin)elisa kit

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1、HumanOmentinELISAKitCatalogNumber.CSB-E09745hForthequantitativedeterminationofhumanomentinconcentrationsinserum,plasma,cellculturesupernates,tissuehomogenates.Thispackageinsertmustbereadinitsentiretybeforeusingthisproduct.IfYouHaveProblemsTechnicalServiceContactinformationPhone:86-27-8758

2、2341Fax:86-27-87196150Email:tech@cusabio.comWeb:www.cusabio.comInordertoobtainhigherefficiencyservice,pleasereadytosupplythelotnumberofthekittous(foundontheoutsideofthebox).1PRINCIPLEOFTHEASSAYThisassayemploysthequantitativesandwichenzymeimmunoassaytechnique. Antibodyspecificforomentinhas

3、beenpre-coatedontoamicroplate.Standards andsamplesarepipettedintothewellsandanyomentinpresentisboundbythe immobilizedantibody.Afterremovinganyunboundsubstances,a biotin-conjugatedantibodyspecificforomentinisaddedtothewells.After washing,avidinconjugatedHorseradishPeroxidase(HRP)isaddedtot

4、hewells. Followingawashtoremoveanyunboundavidin-enzymereagent,asubstrate solutionisaddedtothewellsandcolordevelopsinproportiontotheamountof omentinboundintheinitialstep.Thecolordevelopmentisstoppedandthe intensityofthecolorismeasured.DETECTIONRANGE1.56pg/ml-100pg/ml.SENSITIVITYTheminimumd

5、etectabledoseofhumanomentinistypicallylessthan0.39pg/ml.Thesensitivityofthisassay,orLowerLimitofDetection(LLD)wasdefinedasthelowestproteinconcentrationthatcouldbedifferentiatedfromzero.ItwasdeterminedthemeanO.Dvalueof20replicatesofthezerostandardaddedbytheirthreestandarddeviations.SPECIFI

6、CITYThisassayhashighsensitivityandexcellentspecificityfordetectionofhuman omentin.Nosignificantcross-reactivityorinterferencebetweenhumanomentin andanalogueswasobserved.Note:Limitedbycurrentskillsandknowledge,itisimpossibleforustocomplete thecross-reactivitydetectionbetweenhumanomentinand

7、alltheanalogues, therefore,crossreactionmaystillexist.2PRECISIONIntra-assayPrecision(Precisionwithinanassay):CV%<8%Threesamplesofknownconcentrationweretestedtwentytimesononeplatetoassess.Inter-assayPrecision(Precisionbetweenassays):CV%<10%Threesamplesofknownconcentr