三聚氰胺的遗传毒效应及牛奶中三聚氰胺快速检测方法研究

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1、melaminefromdispersiontoaggregationrapidly,alongwiththecolorchangefromredtoblue(orpurple).Aftertheoptimizationofreactionconditions,thewholeanalysisonlyneedsabout20minincludingthesimplepretreatmentwedesigned.Theanalysiscanbeobservedbythenakedeye,andsimpletooper

2、atewithoutsophisticatedinstruments.Intherawmilk,thepresentlimitofdetectionformelamineis1μMbynakedeyesanddownto375nMbytheinstument.Furthermore,weexploretheinteractionprinciplebetweenmelamineandcitrate-stabilizedAuNPsinthiswork;establishthefoundationformodifying

3、theAuNPsandoptimizingthesystemofAuNPs-melamineinthefuture.Inthispaper,wealsoreportarapidandsensitivecolorimetricsensorformelaminedetectioninmilkbytheaptamerbasednanoparticles(AuNPs).AptamercanabsorbonthesurfaceofAuNPsandelectrostaticallyinteractswithPoly(diall

4、yldimethylammoniumchloride)(PDDA),whichprotectsAuNPsfromaggregating.Theintroductionofmelaminewouldformamelamine-aptamercomplexbyhydrogenbonds,thusthefollowingcationicpolymercouldaggregateAuNPsandcausearemarkablechangeincolor.Accordingtothis,melaminecanbedeterm

5、inedbynakedeyesandmeansofabsorbance.Thissensorisselectivefordetectionofmelamineinmilksamplesandhasalimitofdetectionof34nM.Sinceaptamer-modifiedAuNPsismorestabilizedandselectivethanunmodifiedAuNPsinthesensorofmelamine,wehopethatthisdetectionmethod-buildingproce

6、sswillbehelpfulforthedetectionofothersmallmolecules.Keywords：melamine，genetictoxicity，AuNPs，aptamer，detectionVIII万方数据目录摘要...........................................................................................................................................

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8、............................XII缩略表..........................................................................................................................................XIV第一章绪论