三聚氰胺的遗传毒效应及牛奶中三聚氰胺快速检测方法研究

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1、melaminefromdispersiontoaggregationrapidly,alongwiththecolorchangefromredtoblue(orpurple).Aftertheoptimizationofreactionconditions,thewholeanalysisonlyneedsabout20minincludingthesimplepretreatmentwedesigned.Theanalysiscanbeobservedbythenakedeye,andsi

2、mpletooperatewithoutsophisticatedinstruments.Intherawmilk,thepresentlimitofdetectionformelamineis1μMbynakedeyesanddownto375nMbytheinstument.Furthermore,weexploretheinteractionprinciplebetweenmelamineandcitrate-stabilizedAuNPsinthiswork;establishthefo

3、undationformodifyingtheAuNPsandoptimizingthesystemofAuNPs-melamineinthefuture.Inthispaper,wealsoreportarapidandsensitivecolorimetricsensorformelaminedetectioninmilkbytheaptamerbasednanoparticles(AuNPs).AptamercanabsorbonthesurfaceofAuNPsandelectrosta

4、ticallyinteractswithPoly(diallyldimethylammoniumchloride)(PDDA),whichprotectsAuNPsfromaggregating.Theintroductionofmelaminewouldformamelamine-aptamercomplexbyhydrogenbonds,thusthefollowingcationicpolymercouldaggregateAuNPsandcausearemarkablechangeinc

5、olor.Accordingtothis,melaminecanbedeterminedbynakedeyesandmeansofabsorbance.Thissensorisselectivefordetectionofmelamineinmilksamplesandhasalimitofdetectionof34nM.Sinceaptamer-modifiedAuNPsismorestabilizedandselectivethanunmodifiedAuNPsinthesensorofme

6、lamine,wehopethatthisdetectionmethod-buildingprocesswillbehelpfulforthedetectionofothersmallmolecules.Keywords：melamine，genetictoxicity，AuNPs，aptamer，detectionVIII万方数据目录摘要...............................................................................

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