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1、原发性肝细胞癌HBsAg、Fas和PCNA抗原的表达及其意义论文姜润德宗宁李占元王占民【摘要】目的：通过检测原发性肝细胞癌（HCC）及其癌周肝组织HBsAg、Fas抗原和增殖细胞核抗原（PA）的表达，探讨其表达的规律和意义以及HBV感染与HCC的相关关系，HCC发生的机制。方法：对52例肝癌标本及42例癌周肝组织应用免疫组织化学方法检测HBsAg、Fas和PA的表达，并结合血清甲胎蛋白（AFP）水平进行分析。结果：癌周肝组织高度表达HBsAg和Fas，阳性率均达78.6%，癌组织表达阳性率分别为21.2%和30.8%，阳性表达仅限于分化良好者，差异有统计学意义（P均＜0.005）。癌组织和癌
2、周组织HBsAg和Fas的表达具有相关性。癌组织的PA标记指数（54.8±25.9）.freelaandtheirsignificanceinunderstandingHBVrelatedhepatocarcinogenesis【ABSTRACT】Objective:ToinvestigatetheexpressivecharactersandtheirsignificancebydetectingtheexpressionsofHBsAg,Fasandproliferatingcellnuclearantigen(PA)inbothhepatocellularcarcinoma(HCC)a
3、ndtheirsurroundingnoncanceroustissues.Thecorrelationbetechanismofhepatocellularcarcinogenesismunohistochemistryfrom52casesofhepatocellularcarcinomaasAFPlevelsongHCCpatients,theaveragePALIofHBsAgpositiveonesostPApositivecellsarenucleusovalshapedsmallcellsostlyfoundintheperilobuleareas.Conclusion:Asu
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6、.北京：人民卫生出版社，1997.420425.［5］ToshkovI,ChisariFV,BannaschP.HepaticpreneoplasiainhepatitisBvirustransgenicmice［J］.Hepatology,1994,20(5):11621172.［6］MurakamiS.HepatitisBvirusXprotein:amultifunctionalviralregulator［J］.JGastroenterol,2001,36(10):651660.［7］BirrerRB,BirrerD,KlavinsJV.Hepatocellularcarcinoma
7、andhepatitisvirus［J］.AnnClinLabSci,2003,33(1):3954.［8］ArbuthnotP,Kea［J］.IntJExpPathol,2001,82(2):77100.［9］JakubczakJL,ChisariFV,MerlinoG.SynergybetinggrowthfactoralphaandhepatitisBvirussurfaceantigeninhepat
