小鼠未成熟卵母细胞染色质构型及其和体外成熟能力和发育能力关系的研究

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1、StudiesonchromatinconfigurationofunmaturedmouseoozeanditsrelationshipwiththemeiosisanddevelopmentcompetenceAbstractTherearemoreevidenceshowthatvafiouscstageofantra]follicularoncytebeforematurationcultureinvitro．firstreflectedonthechromminconfigurationofthenucleus，maya

2、ccountfor慨observedflawofinvitromaturationsyslelm。Inthisexperiment．westudiedthechromatinconfigurationchangesduringoogancsisanditsrelationshipwithovulation、meioticcompetence、fertilizationandpreimphntafiondevelopment．n七resultsa托越follows：1)Therearefourtypeschromatinconfig

3、ur蝴uninantralfolliculmooc难isolatedadultmouse．neyarenamedSN、NSN、pSN、pNSN．；SN、NSNtypeoocymismo∞frqncncythanpSN、pNSNtypeoncyte；2)TheappearanceofSN--ooeyteisfirstobunvedin17--day-oldmouse,thera把ofSN-oocyteissignificantly．ma℃asedwhenthellnouseagereachedlgand19days；3)Oocyte

4、indiameterbetween20Innand60)uuisfoundtobeNSN--tmcyte，butwhenOOCyteindiameterreached701am,SN-oocyteisfirstfoundandticrateofSN-oocyteisincreasedaStheOOCytcdiameterreached70---80¨m；11嘲℃aremorepSN和pNSN-oocytewhentheoogyeindiameterissmallerthanthatof70lain；4、PMSGinjectiona

5、ppearedtoinduceanincreaseofSN--ooeyleindiameter70-gOpmandaodecreaseofNSN-oocyteindiameter60^101,tm；ButhCGinjectionindHOgⅫdecreaseofSN—oocyteandbacktOasituationpriortoPMSGinjecfion．5、BothSN．．oocyteandNSN-oocyteisolatedfromarrmdfollicular锄undergoGVBDandprogresstouptomet

6、aphaseILButthemeioticc叽p咖nceofSN-oncyteishigherthanthatofNSN·oocyte；61Thefertilizationrateisnotsigdificant／ydifferentinbothgroupsofm谢删oncytederivedfromSN—andNSN-oocyte；Bmthe2-celldevelopmentrateofSN-occyteishigherthanthatofNSN-oocyte；71TherateofSN—oocytcinantra／follic

7、ularisnotsignificantlydifferentbetwom6．8weeksoldand30-32woeksoldmouse；8)Withageing,therateofSN-oocytcisolatedfromanlralfollicularisincreased．Keyword：chromatinconfiguration，GV-stageooeyte，moose．2．Postgradua恤：YiYalingAdvisor：．Prof．HaoYanhong一旦兰堡尘墨圭塑塑墨塑塞銎鱼垩塑皇丝墨兰堕苎些垄：茎!堕查

8、叁墨堕堡塞壁主笙苎前言胚胎工程操作如胚胎体外生产、胚胎克隆等研究所需要的大量成熟卵母细胞主要是由卵母细胞体外成熟(1vM)提供的．尽管许多种动物的IVM系统取得了根大的进展，目前的ⅣM系统还并不十分完善，这在猪和马尤为突出．侧如母马卵母细胞体外成熟