1-猴头菇多糖对树突状细胞的免疫调节作用

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1、ProcessBiochemistry48(2013)1402–1408ContentslistsavailableatScienceDirectProcessBiochemistryjournalhomepage:www.elsevier.com/locate/procbioImmunomodulatoryeffectsofpolysaccharidesisolatedfromHericium erinaceusondendriticcellsଝShyang-ChwenSheua,∗,YingLyua,Meng-ShiouLeeb,Jai-HongC

2、hengc,∗∗aDepartmentofFoodScience,NationalPingtungUniversityofScienceandTechnology,No.1,ShuehfuRoad,Neipu,Pingtung,Taiwan bDepartmentofChinesePharmaceuticalSciencesandChineseMedicineResources,ChinaMedicalUniversity,Taichung,Taiwan cDepartmentofNursing,Shu-ZenJuniorCollegeofMedici

3、neandManagement,Kaohsiung,TaiwanarticleinfoabstractArticlehistory:Received10January2013Receivedinrevisedform7June2013Accepted8June2013Availableonline15June2013Keywords:HericiumerinaceusBioactivepolysaccharides␤-GlucanDendriticcellTH1immuneresponseFunctionalfoodHericiumerinaceus(

4、H.erinaceus;HE)polysaccharides(HE-PS)havebeenshowntohaveimmunomodula-toryactivity.Wefoundthatthebioactivecomponentsof␤-glucanderivativesconsistedof20%inHE-PS.WeusedananalyticplatformforinvestigatingtheeffectsofHE-PSonthematurationofratdendriticcells(DCs),whicharederivedfromratbo

5、nemarrowhematopoieticcells(BMHCs).Theresultsshowedthattreatmentwith50␮g/mLHE-PSchangedthemorphologyoftheDCstoanactiveforminparallelwithasignificanttwofoldincreaseinMHCclassIIandCD80/86surfaceantigenscomparedtothecon-trol.Furthermore,endocytosisbytheDCswassignificantlyreducedatthes

6、amedosage.IL-12,IFN-␥andIL-10cytokinesecretionwassignificantlyincreasedby2.7,1.5and1.6-fold,respectively,comparedtothecontrolaftertreatmentwith50␮g/mLofHE-PS.ThisstudyusedapoweredanalysisplatformtoshowthatHE-PSinducesDCsactivationandmodulatestheTH1immuneresponse.Thus,HE-PShaspote

7、ntialasanimmunopotentiatingagentthatcouldbefurtherdevelopedinthehealthfoodindustry.©2013ElsevierLtd.Allrightsreserved.1.IntroductionMushroomsarewidelyusedintraditionalChinesemedicineandhavealsobeenusedasafoodsourceforthousandsofyears.Anumberofmushroomspecieshavebeenusedasmedicin

8、eorfunctionalfoodsforthetreatmentofdiseasesinCh