pd-1删除后t细胞抗肿瘤作用的实验研究

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1、授予单位代码10089学号或申请号20151067中国图书分类号R730.51HebeiMedicalUniversity博士学位论文学术学位PD-1删除后T细胞抗肿瘤作用的实验研究研究生：赵咫龙导师：蔡建辉教授专业：外科学二级学院：河北省人民医院2018年4月目录中文摘要·······································································································1英文摘要······

2、·································································································4英文缩写·······································································································8研究论文PD-1删除后T细胞抗肿瘤作用的实验研究引言···················

3、···················································································11第一部分CRISPR/CAS9载体构建慢病毒转染T细胞删除PD-1前言······································································································14材料与方法······················

4、····································································15结果······································································································28附图·········································································

5、·····························30附件······································································································37讨论······································································································54小结······

6、································································································56参考文献······························································································57第二部分PD-1删除T细胞体外抗肿瘤的实验研究前言····························

7、··········································································62材料与方法··························································································63结果············································································

8、··························73附图······································································································74讨论······································································································78小结·