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ID:23282510
大小:2.33 MB
页数:55页
时间:2018-11-06
《银丹心脑通软胶囊抗动脉粥样硬化作用的实验研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、万方数据中国中医科学院二O一一级硕士研究生学位论文(1)Y-XC的最大无毒浓度为500lag·mL~,将250Pg·mL~、125gg·mL~、62.5gg·mL。1三个浓度作为YXC的实验浓度;7INF一0【的造模浓度选择60ng’mL~,作用时间为24h;(2)对细胞生存率的影响YXC孵育24h后可明显升高Ox.LDL诱导的HUVEC的存活率(p<0.05),具有明显的量效关系:YXC孵育24h后可明显升高TNF.CL诱导的HUVEC的存活率(p2、效关系明显。(3)对粘附分子分泌的影响YXC可显著降低Ox—LDL诱导的HUVEC损伤模型中PECAM.1的含量(p<0.05)和ICAM—l的含量(p<0.05);YXC可显著降低TNF一0【诱导的HUVEC损伤模型中ICAM.1、PECAM.1含量(p<0.05);(4)对Ox.LDL诱导的HUVEC细胞凋亡的影响YXC可降低Ox—LDL诱导的HUVEC模型中细胞的凋亡率,抑制细胞凋亡。结论1.YXC与游泳联合应用具有预防大鼠AS的作用,其作用环节可能与改善血液流变学指标、降低血脂水平、保护血管3、内皮功能、抑制血管重构有关。2.YXC对Ox.LDL和TNF.。【诱导的HUVEC损伤具有保护作用,其机制可能与减少1CAM一1、PECAM.1的分泌,抑制细胞凋亡有关。关键词:银丹心脑通软胶囊;动脉粥样硬化;运动;血液流变学;血脂;内皮保护;血管重构;粘附分子万方数据银丹心脑通软胶囊抗动脉粥样硬化作用的实验研究ResearchobjectABSTRACTAtherosclerosis(Atherosclerosis,AS)isthecommonpathologicalbasisofcardiova4、sculardisease,suchasangina,myocardialinfarction,strokeandSOon.Itisoneofthemostseriouspathophysiologicalprocessendangeringlifeandhealth.Anditwasknownas‘‘leadingkiller”indevelopedcountriesanditsincidencerateinourcountryshowedatrendofrisinginrecentyears.T5、hepathogenesisofASwascomplex,fromthemiddleofthe19thcenturytothe1980s,Duguid,Virchow,Steinbergputsforwardthetheoryofthrombosis,lipidinfiltrationtheory,oxidationtheoryrespectivelytoexplainstheformationmechanismoftheASfromdifferentangles.Rossadvancesinjur6、y—reactiontheorywhichstatesthatthearterialintimalinjurywasinitialpartofASin1976.ThistheoryhasacquiredageneralcommonunderstandingathomeandabroadinrecentyearsalongwiththedeepresearchofthepathogenesisofAS.FromtheperspectiveoftraditionalChinesemedicine,ASi7、sankindofsystemicdiseasewithqiandbloodstasis,phlegm,meridiansblockage.Itisoftentreatedwithpromotingbloodcirculationandremovingbloodstasis,whichisacomprehensiveregulativeprocessembodyingtheadvantagesofmulti—target,multi—signalpathwaybyChinesetraditional8、medicinecompound.YindanXinnaotongcapsule(7xc)iscomposedofGinkgobiloba,RadixSalviaemiltiorrhizae,Gynostemmapentaphyllum,Erigeronbreviscapus,Allium.sativanaL.var.ViviparumRegel,Panaxnotoginseng,CrataeguspirmatifidaBgeandBorneolumsynthetic
2、效关系明显。(3)对粘附分子分泌的影响YXC可显著降低Ox—LDL诱导的HUVEC损伤模型中PECAM.1的含量(p<0.05)和ICAM—l的含量(p<0.05);YXC可显著降低TNF一0【诱导的HUVEC损伤模型中ICAM.1、PECAM.1含量(p<0.05);(4)对Ox.LDL诱导的HUVEC细胞凋亡的影响YXC可降低Ox—LDL诱导的HUVEC模型中细胞的凋亡率,抑制细胞凋亡。结论1.YXC与游泳联合应用具有预防大鼠AS的作用,其作用环节可能与改善血液流变学指标、降低血脂水平、保护血管
3、内皮功能、抑制血管重构有关。2.YXC对Ox.LDL和TNF.。【诱导的HUVEC损伤具有保护作用,其机制可能与减少1CAM一1、PECAM.1的分泌,抑制细胞凋亡有关。关键词:银丹心脑通软胶囊;动脉粥样硬化;运动;血液流变学;血脂;内皮保护;血管重构;粘附分子万方数据银丹心脑通软胶囊抗动脉粥样硬化作用的实验研究ResearchobjectABSTRACTAtherosclerosis(Atherosclerosis,AS)isthecommonpathologicalbasisofcardiova
4、sculardisease,suchasangina,myocardialinfarction,strokeandSOon.Itisoneofthemostseriouspathophysiologicalprocessendangeringlifeandhealth.Anditwasknownas‘‘leadingkiller”indevelopedcountriesanditsincidencerateinourcountryshowedatrendofrisinginrecentyears.T
5、hepathogenesisofASwascomplex,fromthemiddleofthe19thcenturytothe1980s,Duguid,Virchow,Steinbergputsforwardthetheoryofthrombosis,lipidinfiltrationtheory,oxidationtheoryrespectivelytoexplainstheformationmechanismoftheASfromdifferentangles.Rossadvancesinjur
6、y—reactiontheorywhichstatesthatthearterialintimalinjurywasinitialpartofASin1976.ThistheoryhasacquiredageneralcommonunderstandingathomeandabroadinrecentyearsalongwiththedeepresearchofthepathogenesisofAS.FromtheperspectiveoftraditionalChinesemedicine,ASi
7、sankindofsystemicdiseasewithqiandbloodstasis,phlegm,meridiansblockage.Itisoftentreatedwithpromotingbloodcirculationandremovingbloodstasis,whichisacomprehensiveregulativeprocessembodyingtheadvantagesofmulti—target,multi—signalpathwaybyChinesetraditional
8、medicinecompound.YindanXinnaotongcapsule(7xc)iscomposedofGinkgobiloba,RadixSalviaemiltiorrhizae,Gynostemmapentaphyllum,Erigeronbreviscapus,Allium.sativanaL.var.ViviparumRegel,Panaxnotoginseng,CrataeguspirmatifidaBgeandBorneolumsynthetic
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