谈述论文翻译1465

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1、谈述论文翻译1465谈述论文翻译1465导读：anti-livercancerdrug.Keyaisamonmalignancyanditsincidenceisrisingrecentlyinthedevelopingorethan600,000deathsTheapoptosisinductioneffectofOligochitosanonhumanhepatocyteandhepatomacellsAbstract.Chitosanoligosaccharide(cso),thedegradationproductsofch

2、itin,areconfirmedhavingag/mloligochitosaneffect24h,thereacellsacellsandlivercellstheIC50g/ml,and3.0-4.0mg/ml.Treatedg/mlCOSfor24h,theapoptosisofhepatomacellsincreasedto76.04%,andtheBcl-2expressionofhepatomacellsreduced,entalbasisfortheclinicaldevelopmentofneaisamonmali

3、gnancyanditsincidenceisrisingrecentlyinthedevelopingorethan600,000deathsareduetohepatocellularcarcinomaeachyear(1).Hepatocellularcarcinomabeestheseventhmostmoncancerotherapyisnotefficientforlivercancerpatients,becauseofdrugresistancethetoxicsideeffects(3).Thereforeitis

4、urgenttofindanoveltherapeuticdrug.Oligochitosan,obtainedbyhydrolysisordegradationofchitin(3),has3-10saccharide(N-acetyl-glucosamineorglucosamine)residues(4).StudyhasbeenindicatedthatOligochitosanhasamunityregulation,anti-tumor,anti-diabetic,antioxidantandliverprotectio

5、n(5,6).Hoorcellsofthisstudyanhepatocyteandhepatomacells,entalbasisfortheclinicaldevelopmentofnovelanti-livercancerdrug.MaterialsandMethodsCellculture.TheChangLivercellsBiochemistydepartmentofDalianMedicalUniversityandSMMC-7721cellsATCC.Bothcelllinesediumsupplementedand

6、antibioticsat37℃,inahumidifiedincubatorediumcontaining0.5,1.0,1.5,2.0,2.5,3.0,3.5and4.0mg/mlCOSg/ml)g/ml)for4h.ThecellsinedultiskanAscentplatereader(1).Hoechst332585谈述论文翻译1465(2)导读：obvious(P>0.05),onlythehigherconcentrations(3.5,4.0mg/ml)ofCOSaffectedChangLivercells

7、grog/ml)significantlyinhibitedtheproliferationofSMMC-7721cells(livercancercells)atlog/ml)ent,thecellslof4%(V/V)formaldehydeat4℃for10min.Then1mlstainingsolutionofHoechst33258inatroomtemperature.Thecellsbyafluorescencemicroscopy.Cell-cycleanalysisandAnnexin-V/PIstaininga

8、ssay.Thecells(1.0×106/ml)2cellcultureflask.ChangLiverandSMMC-7721cellsseededintosixent,thecellsinindarkandanalyzedbyf