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1、TheapoptosisinductioneffectofOligochitosanonhumanhepatocyteandhepatomacellsAbstract.Chitosanoligosaccharide(cso),thedegradationproductsofchitin,areconfirmedhavingawiderangeofphysiologicalfunctionsandbiologicalactivity.Inthisstudy,weexploredtheapoptosisinductioneffectofC
2、OSonlivercancercells.Ourdataindicatedthatwith0.5-2.5mg/mloligochitosaneffect24h,therewasadose-dependenteffect,andtheinhibitionofhepatomacellswassignificantlygreaterthanthelivercells.OnhepatomacellsandlivercellstheIC50was2.5mg/ml,and3.0-4.0mg/ml.Treatedwith2.5mg/mlCOSfor
3、24h,theapoptosisofhepatomacellsincreasedto76.04%,andtheBcl-2expressionofhepatomacellsreduced,whileCaspase-3increased,butitdidnotaffectthetwoproteinsofhepatocytes.Ourresultswillprovideanexperimentalbasisfortheclinicaldevelopmentofnewtoxicsideeffectsofanti-livercancerdrug
4、.Keywords:Oligochitosan,ChangLiver,SMMC-7721,Apoptosis.IntroductionHepatocellularcarcinomaisacommonmalignancyanditsincidenceisrisingrecentlyinthedevelopingworld.Morethan700,000newcasesarediagnosedthroughouttheworldandmorethan600,000deathsareduetohepatocellularcarcinomae
5、achyear(1).Hepatocellularcarcinomabecomestheseventhmostcommoncancerworldwide,andthethirdleadingcauseofcancer-relateddeaths(2).Convertionalchemotherapyisnotefficientforlivercancerpatients,becauseofdrugresistancethetoxicsideeffects(3).Thereforeitisurgenttofindanoveltherap
6、euticdrug.Oligochitosan,obtainedbyhydrolysisordegradationofchitin(3),has3-10saccharide(N-acetyl-glucosamineorglucosamine)residues(4).StudyhasbeenindicatedthatOligochitosanhasawiderangeofbiologicalactivities.Inparticular,Oligochitosanhavebeenreportedtohavethehealthbenefi
7、tssuchasimmunityregulation,anti-tumor,anti-diabetic,antioxidantandliverprotection(5,6).However,theapoptosisinductioneffectofOligochitosanontumorcellswasrarelyreported.TheaimofthisstudywastoexploretheapoptosiseffectofOligochitosanonhumanhepatocyteandhepatomacells,whichpr
8、ovidedanexperimentalbasisfortheclinicaldevelopmentofnovelanti-livercancerdrug.MaterialsandMethodsCellculture.T
