透骨消痛颗粒干预oa早期cox-2和inos表达的实验研究

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1、透骨消痛颗粒干预OA早期COX-2和iNOS表达的实验研究高雪伟摘要：目的通过动物试验探讨透骨消痛颗粒对骨性关节炎早期环氧化酶-2（COX-2）和诱导性一氧化氮合酶（iNOS）表达的影响，研究透骨消痛颗粒在膝骨性关节炎治疗中的相关作用机制。方法新西兰大白兔采用改Hulth法造模，造模成功后随机分成3组：模型组、对照组、实验组，和正常组共4组，分别灌胃给药4周后处死动物取材并观测如下指标：关节腔大体观察，滑膜与软骨组织学观察；关节液内NO、NOS、PGE2含量检测，滑膜软骨中iNOS、COX-2含量检测。结果4周

2、后关节液中NO、NOS含量、PGE2浓度，滑膜和软骨中iNOS、COX-2含量，模型组、对照组、实验组均呈高表达，与正常组比较差异有显著性（P〈0.05）；对照组、实验组与模型组比较差异有显著性（P〈0.05）；对照组与实验组比较差异有显著性（P〈0.05）。结论透骨消痛颗粒可通过抑制COX-2和iNOS在OA早期滑膜和软骨细胞中的表达及其诱导产物PGE2和NO的生成，从而达到控制OA早期炎症、减轻疼痛、缓解症状的作用关键词：透骨消痛颗粒骨性关节炎滑膜软骨COX-2iNOSNOPGE2TheExperiment

3、alResearchaboutgranulaofpenetratingboneremoveingpainintervenetheexpressionofCOX-2andiNOSinearlystageofosteoarthritisXueweiGao,JinshuiZhou,BolingLiuAbstractObjective:Toinvestgatethemechanismofinfluenceaboutgranulaofpenetratingboneremoveingpainintervenetheexpr

4、essionofCOX-2andiNOSinearlystageofosteoarthritisbyanimalsexperiment,toinvestgatemechanismofgranulaofpenetratingboneremoveingpainontreatmentofosteoarthritis.Methods:WedevidedtheNewZealandrabbitsinto3groupsrandomlyafterreplicatedkneeosteoarthritiswithmodifiedH

5、ulthmodelingmcthod:modclgroup.controlgroup，experimentalgroup，withthenormalgroupwere4groupsintotal,wcexecuteallrabbitsaftcrtheywerefedintragasticallyfor4wcckcs.Thenthefollowingdetectionweretakenout:GeneralObservationofarticularcavity;histologicalConversationo

6、fsynoviumandcartilage;contentofNOS，NO，testofiNOSmRNA，concentrationofCOX-2andPGE2withinjoint.ResuIts:Afterfourweekes，therewassignificantcontrast(P<0.05)oftheconcentofNOS,NO,iNOSmRNA,COX-2andPGE2ofmodelgroup,controlgroup，experimentalgroupcomparedwiththenormalg

7、roup;Therewassignificantcontrast(P<0.05)oftheconcentofNOS，NO,iNOSmRNA，COX-2andPGE2ofexperimentalgroupsandcontrolgroupcomparedwiththemodelgroup;Therewassignificantcontrast(P<0.05)oftheconcentofNOS，NO,iNOSmRNA，COX-2andPGE2ofexperimentalgroupcomparedwithcontrol

8、group.ConclusioiuThegranulaofpenetratingboneremoveingpaincancontrolinflammation，relievepainsandalleviatesymptomsinearlystageofosteoarthritisbyinhibitingtheexpressionofCOX-2andiNOSwithincartilage