the total flavanone of longan seed extraction and the identification by ethanol

《the total flavanone of longan seed extraction and the identification by ethanol》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、TheTotalFlavanoneofLonganSeedExtractionandtheIdentificationbyEthanol：HUANGSuoyi,TANGYulian,LIHaini,edicineedicine,longanhasmanycurativeeffectsontheillnessofedicine.Longannucleolusescanalsopreventpain,hemorrhage,hernia,mangeandskindiseases［2,3］.Atthesametime,inlongannuc

2、leoluses,somekindsofalkaloidandfattyoilarealsocontained.Solongancanbepoedicineinfamily,forithasfavorableeffectonstanchingandmakingtheAppropriatesLostArticles.Longannucleolusesoccupy17%ofthegrossakefulluseofitsnaturalresources,longannucleolusesmustbestudiedfurther.Atpresen

3、t,therehasbeenlittlereportabouttotalflavanoneinlongannucleoluses.Inthispaper,thestudyonlongannucleolusesisprovided.　　1Instrumentsandreagents　　1.1InstrumentsKQ5200DBnumericalcontrolultrasonicentlimitedpany);UV-755Bultravioletvisiblespectrophotometer(Shanghaianalysisinstrum

4、entmainplant);ZF-Ithreeusestheultravioletanalyzer(Shanghaitoattendtovillagephotoelectricityinstrumentplant);SHB-IIIultifunctionvacuumpump(Zhengzhougreatentfactory).　　1.2Reagents　　95%Ethylalcohol;SodiumnitriteAR;AluminiumnitrateAR;SodiumhydroxideAR;AluminumtrichlorideAR;Hy

5、drochloricacidAR;AmmoniaacetateAR;MethylalcoholAR;MagnesiumpoalbutylalcoholAR;Standardrutin(Chinesedrugsbiologicalpreparationsexamination);HydrogenperoxidesolutionAR;FerroussulfateAR;SalicylicacidAR.LongansnucleusesoriginatesfromtheGuangxiBaise.　　2Methodsandresult　　2.1Tot

6、alflavanoneingredientsesl95%alcohol.Thereagentsinatedunderloevolumelapproximately,andtransferredtothe25mlmeasuringflask,delutedinationInordertodetectthecontentsoftotalflavanonesintheseedsoflongan,thealuminumionreagentethodofspectrophotometry.　　2.3Determinationoftotalflava

7、none　　2.3.1Theoptimuml5%NaNO2and0.3ml10%Al(NO3)3ed.Theoptimumbythemethodofspectrophotometryfrom420nmto700nmg/ml)0.00,0.50,1.00,2.00,3.00,4.00,5.00mll-flaskrespectively,0.30ml5%NaNO2inutes,0.30ml10%Al(NO3)3inutes,4ml4%NaOHsolution,delutedquietly.Thedataofabsorptionpeaketry

8、at510nm.Table1determinationofabsorbancebyultravioletvisiblespectrophotometer（略）　　2.3.3Determinat