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1、作者：王辉，王岭，易军【关键词】内皮;【Abstract】外周血内皮祖细胞改善肢体缺血的研宄李开宗，凌瑞，孙宝华，李晓军，干细胞；移植；外周血AIM：Toestimatetheeffectofhumanperipheothelialprogenitortationontherepairmentoflimbischemia.clearcellsfromhumaereculturedinM199mithlO%fetalbovinesd4ng/mLbFGFinvitrosofEPCwereanalyzeduringcultureres
2、peclfemoralartery,greiliaccircumflexartnchof15Balb/cnudemuselimbischemia.ThIsmarkedbyCMDilwerdintotheleftlocalmmbs(EPCgroup)andMlectedintotherightlralbloodderivedendcell(EPC)transplanMETHODS:Themononunperipheralbloodwediumsupplementedwerum,20ng/mLVEGFan.Thespecificma
3、rkerbyFCSatday6and14dtively.Thebilateraatsaphenousartery,ery,andmuscularbraicewereligatedtocae6dayadherencecelemultipointinjecteusclesofischemicli99wasmultipointinjocalmusclesofischemiclimbs（M199group）inordertoevaluatethetherapeuticeffect.Sevendaysafteroperation,60Pg
4、FITCUEAwasinjectedviatailhourlater,themicewereheart,liver,1ung,spleen,kidneyandlimbgastrocnemiusweretakenandexaminedwithfluorescencemicroscopy.ThenumberofcapillariesofischemiclimbswasmeasuredbyfactorVlllrelatedantigenimmunohistochemicalstaining.RESULTS：Theculturedcel
5、lsatday6expressedKDR,CD133,CD34andvWF,andthepositiveratesofKDR,ere（土）％,（土）％，（±）%ay.Atthel4thdaydursexpressedKDR,CD34veratesofKDR,CD34andvWFwere（土）％,（±）%vely；thepositivera（土）%.ThenecrosisaryoftheischemiclimprovedmoreobviouslthatintheM199gro叩（PCD133，CD34andvWFwnd（土）%,r
6、espectivelingculture,thecellandvWFandthepositiand（±）%，respectiteofCD133droppedtonddensityofcapillabsofnudemicewereimyintheEPCgroupthan【Keywords】endothelium；stemcells；transplantation；peripheralblood【摘要】目的：研究人外周血来源内皮祖细胞(EPC)移植对改善肢体缺血的作用.方法：人外周血单个核细胞在体外诱导扩增6d和14d后，分别检测其
7、EPC特异性标志的表达，并将荧光染料标记后的培养第6日的贴壁细胞通过缺血局部多点注射移植到后肢缺血的裸鼠动物模型体内，以评价其治疗效果.结果：人外周血单个核细胞经体外诱导分化，培养第6曰的贴壁细胞表达KDR,CD133，CD34和vWF，流式细胞仪检测其阳性率分别为％，％，％和％;培养第14日的贴壁细胞KDR，CD34和vWF的表达阳性率均增高，而CD133表达阳性率明显降低，流式细胞仪检测其阳性率分别为％，％，％和％;移植EPC后裸鼠缺血后肢的坏死情况和毛细血管密度均较对照组明显改善(P【关键词】内皮；干细胞；移植；外周血19
8、97年Asahara等［1］在Science杂志发表论文报道内皮祖细胞(endothelialprogenitorcells,EPC)分离成功，并在体内证明其血管生成的能力.从此EPC受到了研究者的普遍关注，国内外关于EPC的研究方兴未艾［2-3］.骨髓、外周血
