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1、复方褐毛甘西鼠尾对大鼠离体心肌缺血再灌注损伤的保护作用作者：段为钢，李惠兰，张荣平，苏彦宏，郑春兰【关键词】心肌缺血/再灌注损伤；，，保护作用；，，褐毛甘西鼠尾摘要：目的与复方丹参注射液比较，探讨及其在抗心肌缺血再灌注损伤方面替代丹参的可行性。方法采用离体Langendorff缺血再灌注模型，低灌15min复灌30min。实验后检测灌流液中MDA含量及LDH,CK，SOD,GSHPx活性，实验过程全程记录冠脉流量、心率和心肌收缩力。结果复方褐毛甘西鼠尾注射液能显著降低离体缺血再灌注后MDA,CK,LDH水平，升高SOD和GSHPx,且作用强度相似于复
2、方丹参；褐毛甘西鼠尾复方注射液能明显增加冠脉流量，增强再灌注期间的心肌收缩力；此两药均减慢缺血再灌注过程。结论复方褐毛甘西鼠尾有较好的抗心肌缺血再灌注损伤作用，褐毛甘西鼠尾可在抗心肌再灌注损伤方面替代丹参。其机制可能与抗氧自由基损伤有关。关键词：心肌缺血/再灌注损伤；保护作用毛甘西鼠尾TheProtectiveEffectofCompoundedlnjectionMadefromSanqiandHemaoganxishuwEiNativetoYunnanonlsolatedMyocardiallschemia/ReperfusioninRatsAbs
3、tract:Objecticardialprotectionidedinjectionsmadefhemia/reperfusionimodelinvitrowasinttheheartatalowflowtionforl5minatfirsalperfusionfor30mitentsofmalEIcdialdtivitiesoflactatedcreatinekinase(CK)se(SOD),andglutathPx)intheflowofeff1on.ContractileforcdrhythmwererecordeResultsInject
4、ionofreasethecontentsofeactmctiesofSODandheeffectwassimilarnjectionofSPM+PNwaveToinvestigatethenducedbythecompounromSPM+PNduringiscnratsinLangendorffroduced,weperfusedratewithKreb1ssolut,thenfollowednormn.Weexaminedtheconehyde(MDA)andtheacehydrogenase(LDII),,superoxidedismutaio
5、neperoxidase(GSHuentafterreperfusie,perfusionflow,andduringexperiment.SPM+PNwasabletodecMDA,CK,LDH,increasGSH-Pxinvitro,andttothatofSM+PN；Theisabletoenhanceventrce,increasecoronaricalcontractileforyflow,andslackenheartrate.ConclusionThecompoundedinjectionfromSPM+PNpossessescard
6、ialprotectiveeffectagainstischemia/perfusioninjury,whichisnot1esspowerfulthantheinjectionfromSM+PN，andwhichsuggestsSPMcanbealikelyreplacerforSMtoprotectmyocardiumfromischemia/reperfusioninjury.Themechanismsofwhichmaybeassociatedwithdeactivatingoxygenfreeradicles.Keywords：Ischem
7、ia/reperfusioninjury；Protection；S.przewalskiiMaxim.Var.mandarinorumstib.；Panaxnotoginseng(Burk.)中药丹参Salviami11iorrhizaBunge，SM和三七Panaxnotoginseng(Burk.),PN是中国传统的活血化癖■中药，常一起配伍使用用于缺血性心肌病的治疗。前期研究表明:褐毛甘西鼠尾注射液在体内外均能显著抑制ADP、血小板活化因子、花生四烯酸诱导的血小板聚集[1];增加离体心脏冠脉流量；对抗心肌缺血引起的红细胞压积升高，加快血流速度，
8、增加毛细血管开放数目，改善微循环、血液流变学和血流动力学特征[1];减轻心肌缺血时超微结构的病理变化，减少心
