迷走神经刺激对红藻氨酸致癫痫大鼠孤束核内谷氨酸受体和γ-氨基丁酸受体影响

《迷走神经刺激对红藻氨酸致癫痫大鼠孤束核内谷氨酸受体和γ-氨基丁酸受体影响》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、迷走神经刺激对红藻氨酸致癫痫大鼠孤束核内谷氨酸受体和γ-氨基丁酸受体的影响　　【摘要】目的探讨红藻氨酸（KA）致癫痫和迷走神经刺激抗癫痫的作用机制。方法把动物分为KA组、迷走神经刺激＋KA组和对照组。每只大鼠的延髓尾段切片分为5套。第1、2套切片分别入兔抗GluR5或小鼠抗GABAAR血清，用ABC法进行免疫组化染色，观察孤束核内GluR5和GABAAR阳性结构的变化；第3～5套切片分别用于替代对照或Nissl染色。结果（1）对照组动物的孤束核内可见中等密度的GluR5样和GABAA受体β亚单位（GABAARβ）阳性神经元、神经纤维和末梢

2、；（2）KA组动物孤束核内GluR5阳性结构的密度增加，而GABAARβ阳性结构的密度降低；（3）迷走神经刺激（VNS）＋KA组动物孤束核内的GluR5和GABAARβ阳性结构的密度变化与KA组相反，即GluR5阳性结构减少和GABAARβ阳性结构增加。结论孤束核内的谷氨酸受体和GABAA受体在KA致癫痫和VNS抗癫痫过程中可能发挥着重要的作用。　　InfluenceofvagusnervestimulationuponglutamatereceptorandGABAAreceptorinnucleustractsolitaryofthe

3、kainateinducedepilepticrat　　WANGXinZHANGYan　　DepartmentofNeurology，ZhongshanHospital，ShanghaiMedicalUniversity，Shanghai200032．China　　【Abstract】ObjectiveToclarifytheinfluenceofvagusnervestimulation（VNS）uponglutamatereceptor（GluR）andGABAAreceptorpositivestructuresinthenucle

4、ustractsolitary（NTS）ofthekainate（KA）inducedepilepticrat．MethodsAllratsweredividedintoKAgroup，vagusnervestimulation＋KAgroupandthecontrolgroup．Thesectionsofthecaudelmedullaryoblongatafromeachratofeverygroupwerecollectedinto5sets．Thefirstandsecondsetsofthesectionswereusedtop

5、rocessforimmunohistochemicalstainingbyusingspecificrabbitandmouseantibodiesagainstGluR5andGABAARandtoobservethechangesofGluR5andGABAARpositivestructuresintheNTS．ThethirdtofifthsetsofthesectionswereusedforcontroltestsandNissl′sstaining．Results（1）Incontrolgroup，mediumdensit

6、yofGluR5-likeimmunoreactive（GluR5-LI）andGABAAreceptorβchain（GABAARβ）-LIneuronalcellbodies，fibersandterminalswereobservedintheNTS；（2）InKAgroup，thedensityofGluR5-LIpositivestructureswereincreased，whilethedensityofGABAARβ-LIpositivestructuresweredecreased；（3）InVNS＋KAgroup，th

7、echangeswerecontrarytotheKAgroup，showing，thedensityofGluR5-LIpositivestructuresweredecreased，andthedensityofGABAARβ-LIpositivestructuresincreased．ConclusionGlutamateandGABAAreceptorsinNTSmightplayimportantrolesinKAinducedepilepsyandinanti-epilepticeffectsofVNS．　　【Keywords

8、】Epilepsy；Receptors，glutamate；Receptor，GABAA；Vagusnerve；Kainicacid　　近年来，迷走神经刺激（vagusnervestimula