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《5-氮-2-脱氧胞苷联合曲古抑菌素a对人胃癌细胞系mgc-803细胞增殖及runx3基因表达的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、徐州医学院硕士学位论文Effectsof5-Aza-2-deoxycytidineandTrichostatinAonCellProliferationandExpressionofTumorSuppressorGeneRUNX3inMGC-803CellsAbstractObjectiveTostudythecellproliferationandexpressionofTumorSuppressorGeneRUNX3inhumangastriccancercelllineMGC-803treatedbytheDNAmethyltransferase
2、inhibitor(5-Aza-2-deoxycytidine,5-Aza-cdR)andHistonedeacetylaseinhibitor(TrichostatinA,TSA)andtoclarifytheeffectsof5-Aza-CdRandTSAthattheycaninhibitgastriccancercellgrowth,soastoprovidetheoreticalprincipleofgastriccancertreatmentviausingtheDNAmethyltransferaseinhibitorandHistone
3、deacetylaseinhibitor.MethodsWecultivatedgastriccancercelllineMGC-803invitroandmadetheminlogarithmicgrowthphase.Thetrialwasconsistedoffivegroups.(1)blankcontrolgroup:therewasonlyculturemediumandnocellsordrugs.(2)negativecontrolgroup:therewerecells,culturemediumandnodrugs.(3)5-Aza
4、-CdRgroup:24hoursafterinoculation,gastriccancercelllineMGC-803weretreatedwithdifferentconcentrationsof5-Aza-CdR(2.5,5.0,10.0µmol/L).(4)TSAgroup:24hoursafterinoculation,gastriccancercelllineMGC-803weretreatedwithdifferentconcentrationsofTSA(100,200,300ng/ml).(5)5-Aza-CdRandTSAgro
5、up:24hoursafterinoculation,gastriccancercelllineMGC-803weretreatedwithdifferentconcentrationsof5-Aza-CdR(2.5,5.0,10.0µmol/L),andthentreatedwithdifferentconcentrationsofTSA(100,200,300ng/ml)after24hours.Thefinalvolumeofeachholewas150µl.Fourmultipleporeswereusedforeachconcentratio
6、n.Eachgroupwascultivatedintheconditionof37℃and5%CO2.Thereagentsandculturemediumwerereplacedper24hours.AfterMGC-803cellsweretreatedwithdifferentconcentrationsof5-Aza-CdRand/orTSAfordifferentculturaltime,theexpressionofRUNX3mRNAwasdetectedbyRT-PCR,thecellproliferationwasevaluatedb
7、yMTT,thecellapoptosiswasanalyzedbyflowcytometryandthecellmorphologywasobservedthroughinvertedmicroscope.4徐州医学院硕士学位论文Results1.TheexpressionofRUNX3mRNAwasverylowinnegativecontrolgroupanditgraduallyincreasedwiththeconcentrationof5-Aza-CdRandTSAincreasedrespectivelyin5-Aza-CdRgroupa
8、ndTSAgroupdetectedbyRT-PCR.TheRUNX3mRNArelative
