血管祖细胞在动脉粥样硬化发病中的作用PPT课件.ppt

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1、华中科技大学同济医学院病理系阮秋蓉血管祖细胞在动脉粥样硬化发病中的作用VascularProgenitorCellsContributetoPathogenesisofAtherosclerosisAccumulationofsmoothmusclecells(SMCs)playsaprincipalroleinatherogenesisItgenerallyisbelievedthatneointimalSMCsinvascularlesionsoriginatelocallyfromSMCsofthemediallayer.However,severalphenome

2、nacouldnotbeexplainedbythishypothesis.prefaceVeryfewarticleshavedocumentedSMCsmigratingacrosstheinternalelasticlaminafromthemediaintothesubendotheliallayer.Bloodcellsattachtotheluminalsideoftheinjuredarterypriortothedevelopmentofneointimalhyperplasia.Neointimacouldbeformedintheabsenceofme

3、dialcells.SMChyperplasiacouldbepreventedbyblockingchemokinesoradhesionmolecules,whichplayacrucialroleinrecruitingbloodcellsbuthavenoeffectonmigrationandproliferationofdifferentiatedSMCs.NeointimalSMCsarequitedistinctfrommedialSMCsinphenotypeandgeneexpressionpatterns.SomeoftheSMC-likecells

4、invascularlesionsarederivedfrombloodcellsratherthanfrommedialcells.HypothesisExpressvariousmarkersforSMCs心脏移植Theseresultsindicatedthatthemajorityoftheneointimalcellsderivedfromtherecipientcells,butnotfromthemedialcellsofdonororigin.源于受者细胞骨髓移植Theseresultsindicatedthatbonemarrowcellsgiveris

5、etovascularcells,therebycontributingtoarterialremodelingaftermechanicalinjury.源于骨髓细胞TheseresultsindicatethatbonemarrowcellsgiverisetoSMC-likecellsobservedinhyperlipidemia-inducedatheroscleroticplaques.高脂致AS斑块中SMC样细胞源于骨髓骨髓多能细胞（Pluripotentcells）造血干细胞（hematopoieticstemcells，HSCs)间叶干细胞（mesenc

6、hymalstemcells）HSC(c-Kit,Sca-1,Lin)wasisolatedfromthebonemarrowofLacZmice3,000cellswereinjectedintolethallyirradiatedwild-typemice(LacZ)-.Fourweeksafterbonemarrowreconstitution,thefemoralarteriesoftherecipientmicewereinjuredmechanicallyAt4weeks,theinjuredarteriesshowedneointimalhyperplasi

7、a.TheneointimaandthemediacontainedmanyLacZ-positivecells.SomeLacZpositivecellsexpressedα-SMA.SomeLacZpositivecellsexpressedCD31.Thesefindingsindicatethatthec-Kit,Sca-1,LinfractionofbonemarrowcellshasthepotentialtodifferentiateintoeitherSMCsorECs,therebycontributingt