骨水泥及prolene网在犬骨性胸壁缺损模型修复与重建中的实验研究

《骨水泥及prolene网在犬骨性胸壁缺损模型修复与重建中的实验研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、TheexperimentalstudyofbonecementwithprolenenetintherepairandreconstructionofchestwalldefectindogmodelsAbstractObjectiveOnthebasisofscientificexperimentaldataobtained,weevaluatethephysicalcharacteristicofthebonecement,Prolenenetwithbonecement"sandwich"artificialcompositematerials.Wealsoevaluateadjace

2、nttissuethermaldamageofbonecementinducedbytheexothermicreactionintheprocessofrepairandreconstructionofthechestwall,andactivelyexplorethepreventionfromthethermaldamage.Methods1.Thecompressivestrengthtest:Accordingtotheclinicalapplicationofartificialmaterials,theexperimentwasdividedintothreegroups,nam

3、elypigbiologicalribgroup,bonecementribgroup,prolenenetwithbonecement“sandwich”compositeribgroup.Thegroupsweresubjectedtocompressivestrengthtests.2.Toevaluateexothermicreactiondamageofthebonecementforthetissueofexperimentalanimal,andevaluatetheeffectofhypothermicnormalsalineperfusiononpreventingtherm

4、alinjury.Accordingtotheexperimentalpurpose,4mongreldogswererandomlydividedintothermalinjurygroupandperfusionthermalinjuryprotectiongroup,afterthoracotomy.Inthechestwallandbilateralribsofthedogs,aartificiallargechestwalldefectmodelatthesizeof12cm×6cmwasmade.Twopiecesof15cm×15cmprolenenetswith60gbonec

5、ementinthe"sandwich"methodwasusedfortherepairandreconstructionforthechestwalldefectofexperimentaldogs.Thethicknessofbonecementprosthesischestwallwas10mm.Inthethermalinjurygroup,thedogs4万方数据didn'tnotacceptanyheatinjurypreventionmeasuresTopreventthethermaldamageofadjacenttissuesinducedbyexothermicreac

6、tionintheprocessofmouldingonecement,weconstructednormalsalineperfusioncirculationsystem,inwhichweutilized25℃normalsalinecontinuousperfusioncirculationataspeedof500mlperminutetomatchthecoolingeffect.Finally,muscletissuesamplesofthethermaldamagegroupandthermaldamageprotectiongroupwerestainedwithHEandp

7、hosphotungsticacidhaematoxylin.Theheatshockprotein70wasalsodetectedbyimmunohistochemistryandtransmissionelectronmicroscopic.3.Statisticalanalysis.ThedatawereanalyzedbySPSS18.0statisticalsoftware.Theme