大鼠实验性胃溃疡自愈期间肠嗜铬样细胞的变化.doc

《大鼠实验性胃溃疡自愈期间肠嗜铬样细胞的变化.doc》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、大鼠实验性胃溃疡自愈期间肠嗜铬样细胞的变化作者：何美蓉，张绍荣，宋于刚，陈学清【关键词】胃溃疡Changeinenterochromaffiniikecellsduringhealingofexperimentalgastrieulcerinrats【Abstract1AIM：Toinvestigatethemorphologicandfunctionalchanginlike(ECL)cellsduperimentalgastriculcerinrats.METHODSdulcerswerecausedbicacidtothe

2、serosalorfaceoftheratgastenttimepointsdurinells,histidinedecaexpressionandhistadiedusingimmunohistochemicalstainingionpolymerasechainmunoassay,respectiaredwiththatincontesinenterochromaffringthehealingofex:Aceticacidinduceyapplicationofacetsurfaceoftheanteriricbodies

3、.Atdiffergulcerhealing,ECLcrboxylase(HDC)mRNAminecontentwerestu,reversetranscriptreactionandradioimvely.RESULTS：Compandfunctionalchanginlike(ECL)cellsduperimentalgastriculcerinrats.METHODSdulcerswerecausedbicacidtotheserosalorfaceoftheratgastenttimepointsdurinells,hi

4、stidinedecaexpressionandhistadiedusingimmunohistochemicalstainingionpolymerasechainmunoassay,respectiaredwiththatincontesinenterochromaffringthehealingofex:Aceticacidinduceyapplicationofacetsurfaceoftheanteriricbodies.Atdiffergulcerhealing,ECLcrboxylase(HDC)mRNAminec

5、ontentwerestu,reversetranscriptreactionandradioimvely.RESULTS：Comprolgroup,thepercentageandthemeangraycellsingastricmucoasedimmediatelyondday6.TheybegantoinurnedtotheinitiallCmRNAexpressioningrratsdecreasedondaay6,subsequentlyrostnormalizedonday9ntingastricmucosaoeas

6、ed,lowestonday6n.CONCLUSION：ECLcteintheregulationovalueofHDCpositivesaofulcerratsdecreayl,mostmarkedlyoncreaseonday9andretevelsondayl2.TheHDastricmucosaofulceyl,mostmarkedlyondsegraduallyandalmo.Thehistaminecontefulcerratsalsodecrafterulcerinductioellsmaybeparticipaf

7、ulcerhealingbyweakeningthefunctionofhistaminesynthesis.【Keywords】gastriculcer；enterochromaffinlikecell；histidinedecarboxylase；histamine【摘要】目的：探讨肠嗜铬样细胞与大鼠实验性胃溃疡自愈的关系.方法：通过制备大鼠乙酸性胃体溃疡模型，采用免疫组化、逆转录聚合酶链反应和放射免疫测定的方法，观察溃疡自愈过程中大鼠胃黏膜内ECL细胞形态、组氨酸脱羧酶mRNA和组胺含量的变化.结果：胃渍疡大鼠胃黏膜内ff

8、l)C阳性细胞率和胞质平均灰度值在制模术后第1日即开始降低，术后第6日迖到最低，随后开始回升，术后第12日基本正常.胃黏膜内HDCmRNA表达在制模术后第1日开始降低，第3日最明显，第6日开始恢复，第9日后接近正常.制模术后胃黏膜内组胺含量也出现下调，以术后第6