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ID:20484995
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时间:2018-10-13
《大鼠实验性胃溃疡自愈期间肠嗜铬样细胞的变化.doc》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、大鼠实验性胃溃疡自愈期间肠嗜铬样细胞的变化作者:何美蓉,张绍荣,宋于刚,陈学清【关键词】胃溃疡Changeinenterochromaffiniikecellsduringhealingofexperimentalgastrieulcerinrats【Abstract1AIM:Toinvestigatethemorphologicandfunctionalchanginlike(ECL)cellsduperimentalgastriculcerinrats.METHODSdulcerswerecausedbicacidtothe
2、serosalorfaceoftheratgastenttimepointsdurinells,histidinedecaexpressionandhistadiedusingimmunohistochemicalstainingionpolymerasechainmunoassay,respectiaredwiththatincontesinenterochromaffringthehealingofex:Aceticacidinduceyapplicationofacetsurfaceoftheanteriricbodies
3、.Atdiffergulcerhealing,ECLcrboxylase(HDC)mRNAminecontentwerestu,reversetranscriptreactionandradioimvely.RESULTS:Compandfunctionalchanginlike(ECL)cellsduperimentalgastriculcerinrats.METHODSdulcerswerecausedbicacidtotheserosalorfaceoftheratgastenttimepointsdurinells,hi
4、stidinedecaexpressionandhistadiedusingimmunohistochemicalstainingionpolymerasechainmunoassay,respectiaredwiththatincontesinenterochromaffringthehealingofex:Aceticacidinduceyapplicationofacetsurfaceoftheanteriricbodies.Atdiffergulcerhealing,ECLcrboxylase(HDC)mRNAminec
5、ontentwerestu,reversetranscriptreactionandradioimvely.RESULTS:Comprolgroup,thepercentageandthemeangraycellsingastricmucoasedimmediatelyondday6.TheybegantoinurnedtotheinitiallCmRNAexpressioningrratsdecreasedondaay6,subsequentlyrostnormalizedonday9ntingastricmucosaoeas
6、ed,lowestonday6n.CONCLUSION:ECLcteintheregulationovalueofHDCpositivesaofulcerratsdecreayl,mostmarkedlyoncreaseonday9andretevelsondayl2.TheHDastricmucosaofulceyl,mostmarkedlyondsegraduallyandalmo.Thehistaminecontefulcerratsalsodecrafterulcerinductioellsmaybeparticipaf
7、ulcerhealingbyweakeningthefunctionofhistaminesynthesis.【Keywords】gastriculcer;enterochromaffinlikecell;histidinedecarboxylase;histamine【摘要】目的:探讨肠嗜铬样细胞与大鼠实验性胃溃疡自愈的关系.方法:通过制备大鼠乙酸性胃体溃疡模型,采用免疫组化、逆转录聚合酶链反应和放射免疫测定的方法,观察溃疡自愈过程中大鼠胃黏膜内ECL细胞形态、组氨酸脱羧酶mRNA和组胺含量的变化.结果:胃渍疡大鼠胃黏膜内ff
8、l)C阳性细胞率和胞质平均灰度值在制模术后第1日即开始降低,术后第6日迖到最低,随后开始回升,术后第12日基本正常.胃黏膜内HDCmRNA表达在制模术后第1日开始降低,第3日最明显,第6日开始恢复,第9日后接近正常.制模术后胃黏膜内组胺含量也出现下调,以术后第6
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