偏头痛风湿夹瘀证动物模型的建立(返修)

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1、偏头痛风湿夹瘀证动物模型的建立与评价罗钢1陈辉2古远云1白雪1杨思进1（1泸州医学院附属中医医院心脑病科2泸州医学院附属中医医院综合•老年病科，646000）摘要：目的：尝试建立并评价偏头痛风湿夹瘀证动物模型。方法：使用人工气候箱风湿刺激加利血平化低5-HT伴局部脑血管痉挛协同诱导建立大鼠偏头痛风湿夹瘀证动物模型，通过观察动物一般生命体征（活动、体重、眼眵、毛发、进食、大便），挠头次数，图像采集、分析技术研究模型大鼠风湿夹瘀证生物表征（舌象RGB）的变化及动物凝血、五羟色胺等，观察模型动物是否符合风湿夹瘀症候特征。结果：利血平组与

2、利血平加风湿造模组大鼠较对照组凝血时间明显缩短，痛阈降低，全血及脑内5-HT含量明显下降（P<0.01），利血平加风湿造模组大鼠眼眵增多，活动减少，进食减少，大便稀溏，舌象RGB值分析，对照组大鼠舌象淡红，利血平组舌象紫暗，利血平加风湿组舌质青灰，3组舌象RGB值有明显差异。结论：使用人工气候箱风湿刺激加利血平化低5-HT伴局部脑血管痉挛协同诱导可建立大鼠偏头痛风湿夹瘀证动物模型，但仍需进一步改进造模评价方法与流程。关键词：风湿夹瘀证偏头痛利血平证候模型中图分类号：Q95-34R255文献标识码：A文章编号：Establishme

3、ntofmigrainewithbloodstasissyndromeanimalmodelofrheumatismChenHui1，LuoGang2，Baixue1Yangsijing1Yuanyun-Gu1（1Geriatrics2DepartmentofCardiology，TheSecondAffiliatedHospitalofLuzhouMedicalCollege）Abstract：Thereisevidencetosuggestthatmigrainerheumatismstasissyndromeisobviou

4、slymuchmorethanothersyndromes.Wehavethereforetriedtoestablishratmigrainerheumatismstasissyndromeanimalmodelwithartificialclimatechestrheumatismstimulation,low5-HTcausedbyreserpinecombinedwithlocalcerebralvasospasm.Reserpineplusrheumatismratmodelwassignificantlyshorter

5、inbloodcoagulationtime,painthreshold,5-HTcontentinbloodandbraincomparedwithcontrolgroups.SignificantdifferencesoftongueRGBvaluecanalsobefoundingroups.Allindexesprovedthatratmigrainerheumatismstasissyndromeanimalmodelwassuccessful.Therefore,thismodelprovidesaplatformfo

6、rexplorationmigrainewiththecharacteristicsoftraditionalChinesemedicine.Objective：Trytoestablishandevaluateamigrainerheumatismstasissyndromeanimalmodel.Method：Theuseofartificialclimatechestrheumatismstimulationwithreserpinelow5-HTwithlocalcerebralvasospasminducedbycomb

7、inationofestablishmentofratmigrainerheumatismstasissyndromeanimalmodel,throughtheobservationofanimallifesigns(activity,weight,gum,hair,eating,stool),imageacquisition,analysisofrheumatictechnologyresearchmodelratswithbloodstasissyndromebiologicalcharacterization(tongue

8、RGB)changesandanimalbloodcoagulation,fivehydroxytryptamine.Result：Reserpinegroupandreserpineplusrheumatismratmodelwassignifi