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时间:2018-10-10
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1、分子细胞遗传学常用技术分子细胞遗传学常用实验技术GeneralLaboratoryGuidelinesA.Beforestarted:Beforeyouenterthelaboratory,makesureyouhavereadandfullyunderstandthelaboratorysafetymanual.Pleasereadthelaboratorymanualbelowandfollowtheguidelinesofeachexperiment.Keepinmindthatalltheguidelinesint
2、hismanualareINGENERALbutnotforeverydetailsteps.Makesureyouhavefullyunderstoodeachstepintheprotocolanditsreason.Pleaseaskyoursupervisorifyouhaveanythingunclear.B.Solutions-Ifyouuseasolutionandthevolumeislow(lessthan500ml),notifythepersonwhomakesit.Donotwaituntilthe
3、reisalmostnoneleft.Ifyouanticipateusingalotofaspecificsolution,youmaywanttomakesomeforyourself.Makesureallsolutionsarelabeledwithyourinitials,dateandthechemicalnameandconcentration.C.Pipettipsandmicrofugetubes-Afteryouuseaboxofpipettips,refillitandplaceitontheshel
4、f.Whenseveralaccumulatetechnicianwillautoclavethemandreturntothedrawer.Keepyourplasticboxofmicrofugetubes.Youcanjustrefillyourboxasnecessary.Don’taccumulatemanypipetboxeswithoutrefillingthemorotherswon’thaveanytouse.D.Autoclave-Donotautoclavewasteitemswithsterilei
5、tems.E.Orders-Ifyouseethatsomethingisrunningloworifyouanticipateusingalotofanitem,notifytheresponsiblepersonandhewillplacetheorder.Donotwaituntiltheitemisgoneoralmostgone-givetheorderinadvance.F.LaminarFlowHood-Makesureyousprayitdownwith70%ethanolbeforeandafteryou
6、useit.Ifthehazardouswasteisfilled-autoclaveit.Donotletitoverfill-thewasteshouldbedisposedofweekly.G.Dishes-Wehaveaverysmallsinksowashyourglasswareeveryday.Washwithsoapandwater.Rinsewithdistilledwater.Whenyouritemshavedried,putthemaway.Don’tletitemsaccumulatenearth
7、esink.Thesamegoesforyourbench-cleanupaftereveryuse.H.Equipment-Ifyouhaveaproblemwithanyequipment,notifytheresponsiblepersonimmediately.Beforeusinganyequipment,askfordirectionsorreadthemanualfirst.572007年8月分子细胞遗传学常用技术A.Pipets-Donotsetpipetsovertheirspecifiedrange.P
8、ipettingsolutionupanddownslowly.Takegreatcarewhenpipetschemicalslikechloroform.Refertothefactorywebsiteforinstructionsonhowtofixbrokenpipetsortheinstruc
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