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《aglent型液相色谱仪操作规程（operation regulation of aglent type liquid chromatograph）》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。
1、aglent1100型液相色谱仪操作规程（Operationregulationofaglent1100typeliquidchromatograph）OperationregulationofAglent1100typeliquidchromatograph1.Objective:toestablishthestandardoperationprocedureofAgilent1100highperformanceliquidchromatography(HPLC)toensuretheaccuracyandreliabilityoftheope
2、ration.2.basis:Agilent1100highperformanceliquidchromatographyinstructions.3.scope:applicabletoAgilent1100typehighperformanceliquidchromatographoperationmanagement.4.responsibility:theuseroftheinstrumentisresponsiblefortheimplementationofthisregulation,andthedirectorofQCisrespo
3、nsibleforsupervisingthecorrectimplementationoftheoperationprocedure.5.text:5.1.operationsteps5.1.1.opensthecomputer,entersthewindowsEnglishoperatingsystem,andrunstheBootpSeverprogram.5.1.2.openliquidchromatographhost5.1.3.turnsonthepowerswitchofeachmodulesuchas1100LCautomatics
4、ampler,pumpandneededdetectorinturn.Aftereachmoduleself-testiscompleted,double-clicktheInstrumentOnlineicon,and1100LConautomaticchromatographyworkstation,andConfigurationdialogbox,selecttheleftcolumnmoduleicon,clicktherequiredadd,youcanentertherightuserightcolumnisnotneeded;the
5、selectedmoduleicon,clickRemove,willbeabletoentertheleftcolumnwithouttheuseof.5.1.4.filterstheneededmobilephaseintothesolventbottle.5.1.5.clickthepumpicon,appeartheparametersettingmenu,selectSetuppump,settheflowrateofFlow5.0ml/min,StopTime,andtheproportionofthemobilephase,turnt
6、hePurgevalvecounterclockwise.ClickthePumpControloption,selecton,clickOK,andstartdegassinguntilthechannelhasnobubbles.5.1.6.openthepumponlineelution,with10%isopropylalcoholsolutioncleaning,ifnecessary,fromthecleaningfluidoutletpipewithasyringepump,sothattheflowrateis3~5drops/mi
7、n..5.1.7.clickPumpControl,selectoff,turnoffthepump,turnclockwiseandturnoffthePurgevalve.5.1.8.clickonthePumpicon,clicktheSetupPumpoption,enterthepumpeditscreen,aStopTime(flowrate,retentiontimetoexceedcomponents),andthecurrentratio,phaseineachchannelofthehighestminimumcolumnpre
8、ssureandcolumnpressure,clickOK".5.1.9.clicktheiconunderthepum
