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1、1β、IL-6促进细菌生长的体外研究344中国感染与化疗杂志2007年10月20臼第7卷第5期ChinJInfectChemother,Oct.2007,Vo1.7,No.5TNF一,IL一1l3,IL一6促进细菌生长的体外研究田素飞,蔡日丹,褚云卓,陈佰义?论着?摘要:目的评价TNF—a,IL一18和II一6促进铜绿假单胞菌,金葡菌和鲍曼不动杆菌体外生长作用.方法将铜绿假单胞菌,金葡菌和鲍曼不动杆菌菌液分别加入含有10,50,1O0,5()0Pg,1,10ngTNF—a,IL一1B,IL一6的RPMI1640培养液,孵育2,4,6,8,16～18h计数;另将中
2、和量单克隆抗体与细胞因子混合后,做细菌定量培养.结果II一6对铜绿假单胞菌,IL-I~I对金葡菌以及IL一18对鲍曼不动杆菌在共作用6h时表现为浓度依赖的生长促进作用;特异性细胞因子中和抗体则可显着抑制细胞因子对细菌生长的促进作用.结论细胞因子对细菌的生长促进作用,为迁延性炎症中细菌增殖的机制提供新的依据;而细胞因子对细菌的生长促进作用可被中和抗体所抑制,将为更有效进行抗菌治疗提供有益参考.关键词:肿瘤坏死因子一a;白细胞介素一18;白细胞介素一6中图分类号:R378.11;378.99文献标识码:A文章编号:1009—7708(2007)05—0344～04I
3、nvitrostudyonbacterialgrowthenhancementinthepresenceofTNF一,IL_lpandIL一6TIANSu—fei.CAIRi—dan,CHUYun—zhuo,CHENBai—yi.(DivisionofInfectiousDiseases,TheFirstAffiliatedHospital,ChinaMedicalUniversity,Shenyang110001,China)Abstract:ObjectiveToconductaninvitrostudyofthegrowthofPseudomonasaer
4、uginosa,StnphfococcMsaureusandAcin—etobacterspp.,andevaluatetheirresponsetovariousconcentrationsoftumornecrosisfactor(TNF)一a,interleukin(II)一18,andII一6.MethodsTomonitorthegrowthofbacteriaincubatedwiththecytokinesTNFa,IL-I8andII一6thatwereaddedtoRPMI1640mediuminvariousconcentrations(10
5、,50,1O0,500Pg,1and10ng)at2,4to6,8and16-18h.Thebacterialconcentrationwasestimatedwhenthemixturesofcytokinesandspecificneutralizingmonoelonalantibodies(MoAbs)werein—eubated.ResultsWefoundthatallthreebacterialspeciesshowedconcentration—dependentgrowthenhancementwhenincubatedwithoneormor
6、etestedcytokines.Blockadebyspecificneutralizingcytokinesignificantlyinhibitedcytokine—inducedgrowth.Whencomparedwithcontrol,the6hgrowthresponsewasmaximalwithIL一113forS￡n矗0cocc"aureusandAcinetobacterspp.,andwithII一6forPseudomonasaeruginosa.ConclusionsInthisstudyweprovideadditionalevid
7、enceforanewlyde—scribedmechanismforbacterialproliferationinthepresenceofexaggeratedandprotractedinflammation.Theeffectthatcyto—kine—inducedgrowthenhancementinhibitedbyspecificneutralizingcytokineMoAbsmaybeusefulforantimierobialtherapy.Keywords:Tumornecrosisfactor—a;Interleukin一18;Int
8、erleukin-6细胞
