基因工程试卷.doc

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1、2006～2007学年第二学期（A卷）一、名词解释（每题4分，5小题，共20分）1．Blotting：Blottingistheprocessofidentityingspecificbandsofintrerestamongstallthebandsboundtothenitrocellulose.Donewithspecificprobesorantibodies.2．Probe：ProbeisaspecificDNAorRNAfragmentwhichcanbindwiththesampleDNAorRNAfordection.3．Cosmid：Plasmidshavebe

2、enconstructedwhichcontainafragmentofλDNAincludingthecossite.Theseplasmidshavebeentermedcosmidsandcanbeusedasgene-cloningvectorsinconjunctionwiththeinvitropackingsystem.4．SDSequence：ThespecialDNAsequencewhichbindsto3`endof16SrRNAandinitiatessynthesisofprotein.5.Inclusionbody:“Inclusionbodies"t

3、ypicallyreferstoaggregatesofproteininbacterialcytoplasm.Inclusionbodiesareclassicallythoughttobecomprisedofmisfoldedprotein二、填空题（每题2分，5小题，共10分）：1．RACE:cDNA末端快速扩增2.ori:复制原点（复制起始位点）3.MCS:多克隆位点4.PCR:聚合酶链式反应5.cccDNA:共价闭合环状DNA三、单选题（每题1分，25小题，共25分）1.Prokaryotesandeukaryotesuseseveralmethodstoregula

4、tegeneexpression,butthemostcommonmethodis() A.translationalcontrolB.transcriptionalcontrol C.posttranscriptionalcontrolD.controlofmRNApassagefromthenucleus2.Whateffectwouldtheadditionoflactosehaveonarepressedlacoperon?()    A.TheoperatorsiteontheoperonwouldmoveB.Itwouldreinforcetherepressiono

5、fthatgene C.Thelacoperonwouldbetranscribed    D.Itwouldhavenoeffectwhatsoever3.CuttingcertaingenesoutofmoleculesofDNArequirestheuseofspecial()A.degradingnucleasesB.restrictionendonucleasesC.eukaryoticenzymesD.viralenzymes4.ADNAlibraryis()   A.ageneralcollectionofallgenessequencedthusfar.B.aco

6、llectionofDNAfragmentsthatmakeuptheentiregenomeofaparticularorganism.C.aDNAfragmentinsertedintoavector.D.allDNAfragmentsidentifiedwithaprobe.5.Aprobeisusedinwhichstageofgeneticengineering?()    AcleavingDNAB.recombiningDNAC.cloningD.screening6.Theenzymeusedinthepolymerasechainreactionis()A.re

7、strictionendonucleaseB.reversetranscriptase   C.DNApolymeraseD.RNApolymerase7.下列对DNA聚合酶的描述哪一种是错误的()A.具有5`-3`外切活性B.具有5`-3`聚合活性C.具有3`-5`外切活性D.具有3`-5`聚合活性8.大肠杆菌mRNA上起始密码子上游的SD序列可与某种RNA的3`端配对,然后启动多肽链合成，这种RNA是（）A.tRNAB.SnRNAC.16sRNAD.23srRNA9.反转录酶