基因工程试卷.doc

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1、2006～2007学年第二学期（A卷）一、名词解释（每题4分，5小题，共20分）1．Blotting：Blottingistheprocessofidentityingspecificbandsofintrerestamongstallthebandsboundtothenitrocellulose.Donewithspecificprobesorantibodies.2．Probe：ProbeisaspecificDNAorRNAfragmentwhichcanbindwiththesampleDNAorRNAfo

2、rdection.3．Cosmid：PlasmidshavebeenconstructedwhichcontainafragmentofλDNAincludingthecossite.Theseplasmidshavebeentermedcosmidsandcanbeusedasgene-cloningvectorsinconjunctionwiththeinvitropackingsystem.4．SDSequence：ThespecialDNAsequencewhichbindsto3`endof16SrRNAa

3、ndinitiatessynthesisofprotein.5.Inclusionbody:“Inclusionbodies"typicallyreferstoaggregatesofproteininbacterialcytoplasm.Inclusionbodiesareclassicallythoughttobecomprisedofmisfoldedprotein二、填空题（每题2分，5小题，共10分）：1．RACE:cDNA末端快速扩增2.ori:复制原点（复制起始位点）3.MCS:多克隆位点4.PCR:聚

4、合酶链式反应5.cccDNA:共价闭合环状DNA三、单选题（每题1分，25小题，共25分）1.Prokaryotesandeukaryotesuseseveralmethodstoregulategeneexpression,butthemostcommonmethodis() A.translationalcontrolB.transcriptionalcontrol C.posttranscriptionalcontrolD.controlofmRNApassagefromthenucleus2.Whateffe

5、ctwouldtheadditionoflactosehaveonarepressedlacoperon?()    A.TheoperatorsiteontheoperonwouldmoveB.Itwouldreinforcetherepressionofthatgene C.Thelacoperonwouldbetranscribed    D.Itwouldhavenoeffectwhatsoever3.CuttingcertaingenesoutofmoleculesofDNArequirestheuseof

6、special()A.degradingnucleasesB.restrictionendonucleasesC.eukaryoticenzymesD.viralenzymes4.ADNAlibraryis()   A.ageneralcollectionofallgenessequencedthusfar.B.acollectionofDNAfragmentsthatmakeuptheentiregenomeofaparticularorganism.C.aDNAfragmentinsertedintoavecto

7、r.D.allDNAfragmentsidentifiedwithaprobe.5.Aprobeisusedinwhichstageofgeneticengineering?()    AcleavingDNAB.recombiningDNAC.cloningD.screening6.Theenzymeusedinthepolymerasechainreactionis()A.restrictionendonucleaseB.reversetranscriptase   C.DNApolymeraseD.RNApol

8、ymerase7.下列对DNA聚合酶的描述哪一种是错误的()A.具有5`-3`外切活性B.具有5`-3`聚合活性C.具有3`-5`外切活性D.具有3`-5`聚合活性8.大肠杆菌mRNA上起始密码子上游的SD序列可与某种RNA的3`端配对,然后启动多肽链合成，这种RNA是（）A.tRNAB.SnRNAC.16sRNAD.23srRNA9.反转录酶