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1、百会穴长留针疗法对智力偏低大鼠学习记忆能力影响的实验研究陈华德，李勇浙江中医药大学，杭州310053【摘要】目的：观察百会穴长留针疗法对智力偏低大鼠学习记忆能力的影响,为临床上治疗智力相对偏低人群筛选出一种有效、方便的治疗方法，并为百会穴长留针疗法治疗智力相对偏低人群提供有效的实验依据。方法：将170只SD大鼠通过Morris水迷宫进行筛选、分组，设立低智对照组、高智对照组、百会穴长留针组和西药对照组四个组。每10天用Morris水迷宫测试一次，观察3次。实验结束后用比色法测海马组织中ChAT、AchE活力。结果：①在Morris水迷宫实验中，观察发现治疗30天后，与低智对照组比较，百
2、会穴长留针组和西药对照组能显著缩短定位航行试验的潜伏期(P<0.01)；②在空间探索试验中，与低智对照组比较，百会穴长留针组和西药对照组能提高跨越原平台位置的次数(P<0.05)；③与低智对照组相比，百会长留针组、西药对照组能升高脑组织ChAT活力，其差异具有统计学意义(P<0.01)；④与低智对照组相比，百会穴长留针与西药对照组均能抑制脑组织中AchE活力（P<0.01，P<0.05）。结论：百会穴长留针可以改善大鼠学习记忆能力，其机制可能是通过提高ChAT的活力，增加Ach含量;降低AchE的活性，减少Ach消耗,进而改善大鼠学习记忆能力。【关键词】百会穴；针刺；长留针法；学习记忆
3、能力；大鼠EffectsofLong-Time-RetainingNeedleatBaihuiontheRatswithRelativeLowIntelligenceoftheLearningandMemoryCHENHua-de,LIYong(ZhejiangChineseMedicalUniversity,Hangzhou,310053,China)【ABSTRACT】ObjectiveObservedtheeffectsoflong-time-retainingneedletherapyatBaihuiontheratswithrelativelowintelligenceofl
4、earningandmemory.Methods170Sprague-Dawley(SD)ratswerescreenedthroughtheMorriswatermaze,setupfourgroup:lowintellectualcontrolgroup,highintellectualcontrolgroup,long-time-retainingneedleatBaihuiandcomparisongroup.Morriswatermazetestwasobserved3timeswithevery10days.Aftertheexperiment,measuredbycolo
5、rimetryacetylcholinetransferase(ChAT),andacetylcholinesterase(AchE)activity.Results①IntheMorriswatermazetest,Comparedthelowintellectualcontrolgroupaftertreatmentfor30days,thelong-time-retainingneedleatBaihuigroupandcomparisongroup,cansignificantlyshortenthelatencyofplacenavigationtest(P<0.01);②E
6、xperimentsinspaceexploration,Comparedthelowintellectualcontrolgroup,thelong-time-retainingneedleatBaihuigroupandcomparisongroupcanincreasethenumberoftimesacrosstheformerplatformlocation(P<0.05);③Comparedthelowintellectualcontrolgroup,thelong-time-retainingneedleatBaihuigroupandcomparisongroup,Ch
7、ATactivityinbraintissuecanbeincreased,thedifferencewasstatisticallysignificant(P<0.01);④Comparedthelowintellectualcontrolgroup,thelong-time-retainingneedleatBaihuigroupandcomparisongroupcandecreasetheAchEactivityinbraintissu
