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ID:33385256
大小:1.94 MB
页数:30页
时间:2019-02-25
《电针百会穴对大鼠脑组织atp和腺苷含量的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、温州医学院硕士学位论文电针百会穴对大鼠脑组织ATP和腺苷含量的影响姓名:戴勤学申请学位级别:硕士专业:麻醉学指导教师:王均炉2012-05-19温州医学院硕士学位论文电针百会穴对大鼠脑组织ATP和腺苷含量的影响中文摘要目的:观察电针刺激大鼠百会穴对脑组织ATP和腺苷含量的影响方法:将128只SD大鼠按随机区组的方法分配到对照组(n=8),假电针组(n=60),电针组(n=60)。假电针组(S)和电针组(E)再根据电针后的观察时点(电针后即刻、15min、30min、60min、120min、150min)分成S
2、O、S15、$30、$60、S120、S150:EO、E15、E30、E60、E120、E150(n=lO,每组)。在电针百会穴后即刻、15min、30min、60min、120min、150min时分别将大鼠断头取脑,采用高效液相色谱法(HPLC)检测大鼠脑组织中ATP、腺苷的含量。结果:E15组:与对照组比较脑组织内ATP含量增加,差异具有显著性(P=O.001);与SEl5组比较ATP含量增加,且差异具有显著性(P=O.009)。E60组和SE60mJn相比ATP含量减少,且差异具有显著性(P=0.039
3、);与对照组相比差异无显著性。E120组:与对照组相比脑组织内腺苷含量增加,差异具有显著性(P<0.001);与SEl20组比较腺苷含量增加,差异具有显著性(P<0.001)结论:电针大鼠百会穴可引起脑组织ATP含量变化,表现为电针15min时脑组织中ATP含量增加,60min后却降低,而且电针后120min时脑组织中腺苷含量明显增加。关键词:腺苷;ATP:电针;高效液相色谱法温州医学院硕士学位论文TheeffectofElectroacupunctureatBaihuiacupointonATPandaden
4、osineinrat’ScerebraltissueAbstractObjection:ObservetheeffectofelectroacuptmcturebaihuiacupointonATPandadenosineinrat’Scerebraltissue.Methods:Observetheeffectofadenosinetriphosphate(ATP)andadenosinecontentinbrainofnormalSprague—Dawley(so)ratsafterreceivedEApr
5、etreatmentbaihuiacupoint.128SDratswasdividedintocontrolgroup(n=8),shamelectroacupuncturegroup(n=60),electroacupuncturegroup(n=60).Then,shamelectroacupuncturegroup(S),electroacupuncturegroup(E)wasdividedintoSO,S15,$30,$60,S120,S150;EO,E15,E30,E60,E120,E150gro
6、up(n=10,pergroup),accordingtothetimepointofpost—electroacupuncture(Omin,15min,30min,60min,120minand150minafterEAbeingfinished)Wegotthebrainsamplesat0min,15min,30min,60min,120minand150rainafterEAbeingfinished.Weusedthehighperformanceliquidchromatography(HPLC)
7、techniquetodetectthecontentofATPandadenosine.Results:ThecontentofATPinbraintissuewasincreasedsignificantlyinE15mingroupcomparedwithSE15group(P=0.001)andcontrolgroup(P=O.009),butinE60groupwasdecreasedobviouslycomparedwithSE30min(P<0.001)andcontrolgroup(P8、1)Conclusion:ThecontentofATPwasincreasedinthetimepoint-after15minbeingfinishedEA,butwasdecreasedinthetimepoint—after60minbeingfinishedEA.While,thecontentofadenosineWasincreasedsignificantlyinthe
8、1)Conclusion:ThecontentofATPwasincreasedinthetimepoint-after15minbeingfinishedEA,butwasdecreasedinthetimepoint—after60minbeingfinishedEA.While,thecontentofadenosineWasincreasedsignificantlyinthe
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