人参皂甙rb1对ad大鼠顶叶皮质β分泌酶及ps1表达的影响

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1、万方数据第17卷第5期2008年10月中国组织化学与细胞化学杂志CHINESEJOURNALoFHI翻【’0C邱EMI卯限YANDC”阳(=Ij哐MIsllWV01．17．No．5OcL2008人参皂甙Rbl对AD大鼠顶叶皮质』3～分泌酶及PS一1表达的影响方欣杨吉平高旭红李兆圣赖红。(中国医科大学基础医学院解剖学教研室，沈阳110001)(摘要)目的探讨人参皂甙Rbl对AD大鼠顶叶皮质争分泌酶及PS-1表达的影响。方法30只健康雄性sD大鼠，随机分为对照组、模型组和治疗组。模型组大鼠胃饲氯化铝同时腹腔注射D半乳糖2个月，对照组大鼠给予等剂量生理盐水，治疗组大鼠于造模结束后腹腔

2、注射人参皂甙Rbl4周。采用刚果红染色法观察老年斑，镀银染色法观察神经原纤维缠结，Westernblot方法、免疫组化方法和图像分析技术对争分泌酶及P孓1的表达进行检测。结果模型组与对照组相比有明显的老年斑及神经原纤维缠结形成，伊分泌酶及PS-1的表达增多(P

3、；阿尔茨海默病(中图分类号)R332．81(文献标识码)AIX)I：10．3870／zgzzhx2008．05．012El’FECI’OFGINSENOsIDERblON步SECRETASEANDI’孓1INPARIETALIX)BEOFADMODELRA髑FangXin，YangJiping，GaoXuhong，LiZhaosheng，LaiHong。(De加rtmentofAnatomy，吼inaMedicalUniversity，Shenyang110001，China)[AbstracOObjec峨ToinvestigatetheeffectofginsenosideR

4、blonp-secretaseandPS-1intheparietallobeofAlzheimerdisease-likerats．Methods30SDratswererandomlydividedintothecontr01group，modelgroupandtreatmentgroup．Ther／lodelwasbuiltwithadministrationofD-galactoseandaluminumchloridefor2momhs．theoontrolgroupwasgiventhesamedoseofsodiumchloride，andthetreatmen

5、tgroupwasgivenginsenosideRblfor4weeksafterthemodelwasbuilt．CongoredstainingWasusedtoobservesenileplaques，silverimpregnationmethodwasusedtOobserveneurofibriUarytangle，andtheexpressionofpsecret弱eandP孓1wasdetectedbyWesternblotandimmunohistochemistrywithimageanalysis．ResultsComparedwiththecontro

6、lgroup，seniorplague(SP)andneurofibrillarytanglewerecleartoheseeninthemodelgroup，andthelevelsofpsearetaSeandPS-1weresignificantlyhi曲er(P．

7、．05)．ConclusionTheexpressionof争secre妇andPS-1areincreasedintheparietallobeofAlzheimerdisease-likerats，butginsenosideRblnormalizestheexpressionandprotectsneuron＆[Keywords]p-Amyloid；p_Secretase；Presenilin-1；GinsenosideRbl；Alzheimerdisease人参皂甙(ginsenos