effects+of+environment+factors+on+initiation+of+sperm+motility+in+sea+cucumber+apostichopus+japonicus+（selenka）

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1、J-OceanUniv．China(OceanicandCoastalSeaResearch)DOI10．1007／s11802—011-1748一YISSN1672—5182，201110(2)：165—169http：／／www．ouc．edu．cn／xbywb／E—mail：xbywb@Dcedu．CHEfectsofEnvironmentFactorsonInitiationofSpermMotilityinSeaCucumberApostichopusjaponicus(Selenka)YULi，SHAOMingyu，BAOZhenmin，HU

2、Jingjie，andZHANGZhifengKeyLaboratoryofMarineGeneticsandBreedingofMinistryofEducation，OceanUniversityofChinaQingdao266003，P．R．ChinafReceivedMarch18，2010：revisedJune1，2010：acceptedSeptember16，2010)@OceanUniversityofChina．SciencePressandSpringer-VerlagBerlinHeidelberg2011AbstractSpe

3、rmofseacucumberApostichopusjaponicusrSelenka)werequiescentinelectrolyteNaClsolutionandartificialseawater(ASW、andnonelectrolyteglucoseandmannito1solutionswhentheosmolalitywaslessthan200mOsmkg～．Thespermstartedtobemotileasaresultofincreasedosmolality,indicatinganosmolality—dependent

4、initiationofspermmotilityinseacucum—ber．AfterabriefincubationinhypotonicNaClandglucosesolutionswithosmolalitiesof200and400mOsmkg一，spermlostpartialmotileability．SpermbecameimmobilizedwhenpHwas6．0inNaC1．glucoseandmannitolsolutions，suggestingthatanH’releaseisinvolvedinspermactivatio

5、n．ThedecreasedpHhadnoeffectonthepercentageofmotilesperminASW，whereasitdelayedthetimeperiodtoreachthemaximummotility(motilityma)．ExtracellularCainelectrolytesolutionswasnotessentialformotilitystimulationbutshortenedthetimeofreachingmotilitymax．WhenCawasmixedinnonelectrolytesolutio

6、nsthespermmotilitywascompletelysuppressed．TheK『channelblocker,quinine，suppressedthespermmotilityinelectrolytesolution，showingapossibleinvolvementofKtransportintheprocess．HighconcentrationdidnotaffectthespermmotilityinNaC1solution，butdecreaseditinASWandalmostentirelysuppresseditin

7、nonelectrolytesolutions．ThedifferenteffectsofpHandinASWandNaClsolutionindicatethatexternalionsmayalsoregulatespermmotility．Keywordsseacucumber；sperm；motility；initiation；environmentfactorsnationtechniquehasbeenSuccessfullyappliedinthefer．tilizationofA．japonicus．However，semenobtain

8、edby1lntroductionspawninginductionisofte