腺病毒介导人转化生长因子β2基因转染诱导骨髓间充质干细胞向软骨细胞的定向分化

《腺病毒介导人转化生长因子β2基因转染诱导骨髓间充质干细胞向软骨细胞的定向分化》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、万方数据中国组织工程研究与临床康复乌争74誊乌争49嬲2010—12—03出版JournalofClinicalRehabilitativeTissueEngmeeringResearchDecember3，2010VoL14，No．49腺病毒介导人转化生长因子132基因转染诱导骨髓间充质干细胞向软骨细胞的定向分化料☆鞠晓东1，敖英芳1，王建全1，邓敏2Adenoviral—mediatedtransferofhumantransforminggrowthfactor-beta2geneinducesdire

2、ctionaldifferentiationofbonemarrowmesenchymalstemcellsintochondrocytesJuXiao—don91，AoYing-fan91，WangJian-quanl，DengMin2Abstract。InstituteofSportsMedicitie．2DepartmentofNeurology,ThirdHospital，PekingUniversity,Beijing100191ChinaJuXiao-dong☆．Doctor,Attendingp

3、hysician，InstituteofSportsMedicine，ThirdHospital，PekingUniversity．Beijing100191，Chinajxdl204@163comSupportedby：theNationalNaturaIScienceFoundationofChina．No30973043+：NaturaIScienceFoundationofBeijingCity．No．7102159*Received：2010-06-29，址cepted：2010—07—30北京大学

4、第三医院，1运动医学研究所，2神经科，北京市100191鞠晓东☆，男，1975年生，山东省昌乐县人，汉族，2004年北京大学毕业，博士，主治医师，主要从事运动创伤和关节软骨损失修复研究。jxdl204@163．com中图分类号：R394．2文献标识码：B文章编号：1673—8225(2010)49-09194-05收稿日期：2010-06-29修回日期：2010-07-30(20100629002N＼／’Q)9194BACKGROUND：Previousstudieshaveshownthattransfor

5、minggrowthfactor-pl(TGF—t31)contributestoproliferationofbonemarrowmesenchymaIstemcells(BMSCs)，andinducesthedifferentiationofBMSCsintochondrocytes．0BJECTIVE：ToinvestigatetheeffectofhumanTGFB2(hTGFB2)transfectiononthedirectionaldifferentiationofBMSCsintoosteo

6、blasts．METHODS：Atotalof16deanmaleLewisratsaged3weekswereselectedforisolationofBMSCs．hTGFp2genewastransfectedintothesecondpassageofratBMSCscultured／nv#rothroughAd—hTGFp2．48hourslater。immunohistochemicaIstaining，reversetranscdption-polymerasechainreac【ionandW

7、esternblottingassaywereusedtodeterminetargetgeneandcartilagespecificprotein-typeIIcollagenandaggrecanintransfectedcells．RESULTSANDCONCLUSION：ApopulationofBMSCswassuccessfullyIsolatedfromadultrats．Theyappearedfibroblast-likelyandcouldstablyproliferateandsubc

8、ulture／nvfro．AftertransfectedwithAd．hTGFp2，thedifferentiationofbonemarrowmesenchymalstemcellstowardschondrocyteswasverifiedbythepositiveresultsoftypeIIcollagenproteinexpressionthroughimmunohistochemist