探讨核心蛋白多糖体外抑制兔晶状体上皮细胞的转分化

《探讨核心蛋白多糖体外抑制兔晶状体上皮细胞的转分化》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、万方数据IntJOphthalmol，V01．9，No．3。Mar．2009www．IJ0．皿Tel：029-8224517283085628Emaii：IJO．2000@163．con·实验论著·探讨核心蛋白多糖体外抑制兔晶状体上皮细胞的转分化张桂兰，霍鸣，张海江作者单位：(443003)中国湖北省宜昌市中心人民医院眼科作者简介：张桂兰，女，住院医师，研究生，研究方向：白内障。通讯作者：霍鸣，男，主任医师，教授．ychuoming@163．coln收稿日期：2008一11-21修同口期：2009—02-17

2、Studytodecorininhibitingtransdifferen-tiationofrabbits"lensepithelialcells／nvitroGui-LanZhang，MingHuo，Hai-JiangZhangDepartmentofOphthalmology，theCentralPeople'sHospital，Yichang443003，HubeiProvince，ChinaCorrespondenceto：MingHuo．DepartmentofOphthalmology，theC

3、entralPeople’sHospital，Yichang443003，HubeiProvince，China．ychuoming@163．tomReceived：2008-lI-2lAccepted：2009-02-17Abstract·AIM：T0investigatetheeffectsofthedecorinonsecretionoftransforminggrowthfactor—p1(TGF-p1)andtheexpressionofa—smoothmuscleactin(a—SMA)inthe

4、primarilyculturedrabbits7LEC／nvitro．·METHODS：Culturedrabbits’Iensepithelialcellsprimarilyinvitro．thenturnedtoplateswhentheynearlyfused．Setupcontrolgroupandexperimentgroup，thecontrolgroupwastraining12dayswithoutdrugs，othersweredecorintreatmentgroup(threeconc

5、entrationgroupsof10，1．0，0．1mg／L)for6days’treatmentaftertraining6days．ELISAusedineachgroupintheculturemediumsuIpernatantofTGF·B1Ievels，statisticsexpressionofa—SMAbyimmunocytochemistryandcomputerimageanalysis．·RESULTS：TheleveIofTGF—B1anda—SMAofthreeexperiment

6、groupswassignificantlylowerthanthelevelofcontrolgroup(P<0．01)．Andimmunocytochemistryfiguresshowedthatexperimentgroup’shypertrophiedcellsandpositivestainingincytoplasmwasmuchlowerthancentreIgroup．Itdemonstrateddecorinhadsignificantinhibitioneffect．Moreover．a

7、．SMAdecreasedaccompanyingtothedropofTGF·p1andthebothwerehighlypositivecorrelated(r=0．995，P<0．01)．·CONCLUSION：BecausedecorininhibitstheactivityofTGF．B1toreducedtheexpressionofa-SMA．·KEYWORDS：posteriorcapsularopacification；decorin；lensepithelialcells；transfor

8、minggrowthfactorB1；a—smoothmuscleactinZhangGL．HuoM，Zhang脚．Studytodeeorininhibitingtransdifferentiationofrabbits’lensepithelialcells／17vitro．IntJOpbthMmo／(auo．iiYaleZaztu"J2009；9(3)：437·439摘要目的：探讨核心蛋白多糖