薏苡仁多糖的分离提取及其降血糖作用的研究

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1、维普资讯http://www.cqvip.com一s第22卷第6期第军大学学报Vl1l22．No657821；00年6月A(AAC．M)EM】AEMEDICINAEMlIJTAR1STERI1AEJun2OOO婶编号：删‘吣∞胆占7／论著薏苡仁多糖的分离提取及其降血糖作用的研究斤2J毪梓辉，周世文，黄林清(第三军医大学附属新桥睚院临床药理基地．重庆400037)提要：门的从薏苡仁中分离提取薏葭仁多糖(Colxan)，并观察其对多种模型小鼠血糖水平的影响法①采用热水提取、低温减压浓缩、氯醋酸法去蛋白、流水透析、phadeG-75柱层析等方法②采用四氧嘧啶肾腺素尾静脉注射建立高血糖小鼠模型，尾

2、静脉取血．血糖仪删定血糖水平。结粜①被提取的薏敬仁多糖是鼠李糖、阿拉伯糖甘露糖泮乳糖和葡萄糖组成，分子量约为1．5×】．产率为0125％；0L]服给予Coixan对正常小鼠无明显降血糖作用：腹腔给予Qxximl剂量在5Orag／kg和100时．能降低正常小鼠、四氧嘧啶糖尿病模型小鼠和肾腺索高血糖小鼠的血糖水平(P<0．05&P<0OI)且呈现一定的量效关系结沧薏苡七．多糖对正常及高血糖模型小鼠均有降血糖作用．其降血糖作用与给药途径有关文献标识码：AExtractionandhypoglycemicactionofapolysaccharidefromtheseedsofcoixlacchr

3、yma-jobivar，ma—yuenXUZi-hui，ZHOUShi—wellftUANGLin—qing(ofⅡIⅢPharmacology，KinqiaoHospital，"UdrdMilita~,MedicalUtiversib,，Chongqing400037，China)Albstraet：ObjectiveTostud~,thehieth0dofextractionofcoixm1．apolysaecharidefromtheseedsofeoixlacch~qna-jabivar，ma-yuenanditshypoglycemicactionit,mice．Methods①

4、111edriedcoixseedswereextractedwithhotwatermidtheextractwasconcen[ratedunderlowerpressuretogetthecrudecoixan．Itsproteincontentwosremovedwithtrichloraceticacid(TCA)．Thenthecnldeeolxanwasdia—lyzedwithcelluhrsetubingsagainstflowingwaterfor7dmidchrommoglaphedoversephedex(；-75w4thacolumnof(0一1)mol／LNaC

5、ltoobtainpurifiedcalxm]．②emodelofdiabetesmellituswanestablishedMthalloxauinjectionthroughthetailveininmicemidthemodeofhype唧ycemiaw4thadrenalineinjet—tloninmicetoo．Bloodsampleswe-recollectedwithtailveinpuncturetodetermineblood~lucaseleve1．Results①Refinedcoixanof0．125％yieldingconsistedofihatllOllSe，

6、arabinase，inillrlnoF~，剐actaseandglucosewithanestimatedmolecularwelghtofl5×10'②OraladministrationofeoixantonormalmiceshewednoobviousactiontodecreaseI)l,vodglucoseleve1．Peritonealadministrationofcoixmlinthedosagefrom50to100n1kgw&sabletodecreasebl,x~luo0selevelsignificantlyinnormalmice，dia_t~tieandhy

7、peyeemiemice(P<0．05toP<0．01)indosage—dependentnlalqilerConclusionCoixauisabletodecreasethebk~xlglucoselevelinnormal，diabeticandhyperglycemicmiceanditshy—poglyeemicactionisdeterminedbytheYOUteofadministration．Key,