syndecan1在结直肠腺瘤和腺癌中的表达变化及其意义

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1、万方数据胃肠病学2008年第13卷第10期Syndecan一1在结直肠腺瘤和腺癌中的表达变化及其意义何雅婧陈烨周永柏陈楚弟王霞林英卓宋于刚幸南方医科大学南方医院消化病研究所(510515)·595·背景：Syndecan．1是一种由硫酸乙酰肝素链和硫酸软骨素链修饰的I型跨膜蛋白多糖，主要表达于上皮细胞表面，与肿瘤的多种生物学行为关系密切。目的：研究Syndecan．1在结直肠腺瘤和腺癌中的表达。探讨其与结直肠腺瘤和腺癌发生的关系。方法：分别采用逆转录聚合酶链反应(RT-PCR)和免疫组化染色检测56例结直肠腺瘤、42例腺癌和20例正常结肠组织中Syndecan．

2、1mRNA和蛋白表达，并分析其与结直肠腺癌临床病理特征的关系。结果：RBPCR和免疫组化染色结果显示Syndecan．1mRNA和蛋白在重度异型增生腺瘤和腺癌中的表达显著低于正常结肠组织和轻、中度异型增生腺瘤(P

3、细胞间黏附破坏在结直肠癌的致癌机制中可能起关键作用。关键词Syndecan-1；结直肠肿瘤；逆转录聚合酶链反应；免疫组织化学ExpressionofSyndecan-1inColorectalAdenomaandAdenocarcinomaanditsSignificanceHEYafing,CHENYe，ZHOUYongbai,CHENChudi,WANGXia,LINYingzhuo，SONGYugang．Inst如uteofDigestiveDisease，NanfangHospital,SouthernMedicalUniversity,Guangzho

4、u(510515)Correspondenceto：SONGYugang，Email：songyg@fimmu．cornBackground：Syndecan—lisatypeItransmembraneproteoglycanmodifiedbyheparansulfateandchondroiticacid．Itismainlyexpressedonthesurfaceofepithelialcells，andiscloselyrelatedtomanybiologicalbehaviorsofcancer．Aims：Toinvestigatetheexpr

5、essionofSyndecan-1incolorectaladenomaandadenocarcinoma，andtoexplOreitsrelationshipwiththeprogressionofcolorectaladenomaandadenocareinoma．Methods：ExpressionsofSyndecan一1mRNAandproteinin56colorectaladenomas．42adenocarcinomasand20normalcolonictissuesweredeterminedbyreversetranscdptasepo

6、lymeragechainreaction(RT-PCR)andimmunohistochemistry，respectively．RelationshipbetweenexpressionofSyndecan一1andclinicopathologicalcharacteristicsofcolorectaladenocarcinomaw嬲analyzed．Results：RT-PCRandimmunohistochemistryshowedthatexpressionsofSyndecan-1mRNAandproteininhishdegreedysplas

7、tieadenomaandadenocarcinomaweresignificantlydecreasedthanthoseinnormalcolonictissues，andlowandmoderatedegreedysplasficadenomaC户之0．05)．Nosignificantdifferenceswerefoundinlowdegreedysplagticadenoma,moderatedegreedysplagticadenomaandnormalcolonictissues．Andtherewaftalsonosignificantdiff

8、erencebetwee