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1、ProtocolofthequalitativepolymerasechainreactionfordetectinggeneticallymodifiedplantcomponentsinedibleoilT~UXf++8tl&%~~*~SRtsfi5IH~B%*6@HA@RS&1#9HSIHX#t#M,GEJ?%HB&$(&Q%~iBHb@)%Bi5~%&;BHT*ERt%Thitgh@@%*ER%BB~H83HRE3W@Hjh34rX#HS%M*oRE$&H#9H511X#t#t:a%M*3H
2、T*6@oGB/T6682j)~g!&SJ#&~#@~Bfj-&(eqvIS03696:1987)SN/T1193S~@~~!&~~~~~SN/T1194~@W~F&%~BB%@MM#I#r~1#2i%SN/T1195kS+ffSBB%SBPCR&8!!2it&SN/T119633R+ffSB&%Z#PCRB@l3%SN/T1197?&%H+ff&BB%SBPCRBM2i&!i-SN/T1199#j%+%&Hfi&%Z#@B!lPCR3%SN/T1202.$t&+%SB&@&%Z#PCRW!!I3%
3、SN/T1204&$hW#$QIFa"a+%SBB%AB$?EfiPCR&B@4$2i%T3U#i~%SHT*h5Ro3.1PCR:polymerasechainreaction,m#&PCR.3.2DNA:deoxyribonucleica~id,NR@@j&@~3.3dNTP:deoxyribonucleosidetriphosphate,ag@%Z@@.3.4dATP:deoxyadeNOSinetriphosphate,%!@.8$%=€#@3.5dCTP:deoxycytidinetrip
4、hosphate,$!glfi%3€#@.,3.6dGTP:deoxyguaNOSinetriphosphate,li#RsffZ@@.3.7dUTP:deoxyuridinetriphosphatel~~Jj25jff:€#@3.8bp:basepair,&Sjt;f.3.9Taq88i:NNAB&88.3.10Tris:tris(hydroxymethyI)aminomethane,3(~PS)$ilaSPj$.,3.11TE:Tris-CI.EDTA%#$&.3.12Lectin:B@@%%l
5、.3.13ZEIN:&$QB$%EQ3.14PE3-PEPcase:phosphoenilpyruvateCarboxylase,@@#€!$&fiBA@ftkII.3.15tRNALeu:B@W%#SiH3.16CaMV35s:35spromoterfromcauliflowermdsaicvirus,*$TE##%EWSic5535sG33.3.17NOS:terminatorofnopalinesynthasegene,fdBB#&-$?&Bi&B%It3.3.18FMV35S135Sprom
6、oterfromamodifiedfigwortmosaicvirus(caulimovirusgroup),x&EWI%t35SB325;b3.3.19NPTII:neomycin-3'-phosphotransferasegene,%S!#-3'-@BS@BSH.3.20BAR:phosphinothricinacet~ltransferasegenefromBacillusamyloliquefaciens,*@IBacillusamyloliquefaciensTB2i%%@ISH3.21P
7、AT,phosphinothricinacetyltransferasegene,@TM2%S$$i@SB.ai3.22GOXtglyphosateoxidoreductasegene,#i'HI#$!,kSEi@SE!l6.1JHB@iC&&H3tBflR%SBr$DNAh3;-@BRaH85SSiH@@EMR%Ac559hNi&B.tftff41C,%S$-&AfgjCLZG(PcR)B*,%R&rMfiISafHDNAER,@BPCRfMBWC,Pl@fB&Ha1PcfiEB$%SSH&330
8、6.2f#W#6.2.1PCR%m9W.6.2.2DNA3WBW.6.2.3B?91#&.6.2.4~SXBIBiWI.6.2.5~@PB5j&W.6.2.6$$i!E~tftEB~b#L,13i%/1?4!&jL4l,,Mini+hBjL4l,.6.2.7fiB%qpC,@B@rft;b)(Ro6.2.8%,A%tk%#6.2.9@J%#L.6.2.10LRSSB*6.2.11@B%*%*6.2.12@B.6.2.13#a@#B:0.5pL.2pL.10p