医药学药学毕业论文 奥曲肽对急性坏死性胰腺炎肺损伤的治疗作用

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1、湖南师范大学本科毕业论文考籍号：XXXXXXXXX姓名：XXX专业：医药学药学论文题目：奥曲肽对急性坏死性胰腺炎肺损伤的治疗作用指导老师：XXX二〇一一年十二月十日　　【摘要】　目的　观察奥曲肽对急性坏死性胰腺炎(acutenecrotizcpancreatitis,ANP)大鼠肺损伤的治疗作用，并探讨其机制。方法　选用大鼠63只,在胰胆管内注射5%牛磺胆酸钠溶液诱导大鼠ANP模型。观察奥曲肽对ANP大鼠肺组织形态及细胞因子变化的影响。结果　奥曲肽治疗组较ANP+NS组肺湿/干重显著降低，PaO2明显提高，肺泡灌洗液中蛋白质含量［奥曲肽组为(339

2、.9±72.5)mg/L］和白细胞计数明显下降或呈下降趋势。同时，血浆内毒素、血清TNFα和NO2-/NO3-水平及肺组织NOS活性显著降低，肺组织血管内皮细胞和导管上皮细胞一氧化氮合酶(NOS)表达下调，肺组织病理学改变减轻。结论　奥曲肽对ANP大鼠并发肺损伤有一定的治疗作用，其机制与其对内毒素、肿瘤坏死因子-α和一氧化氮等炎症介质的调节作用有关。　　Beneficialeffectsofoctreotideonthelunginjuryfollowingexperimentalacutenecroticpancreatitisinrats　　【

3、Abstract】　Objective　Toinvestigatethepossiblebeneficialeffectsofoctreotideonthelunginjuryfollowingacutenecrotizingpancreatitis(ANP)andthemechanisms.Methods　TheANPmodelofratwasestablishedbyinjectionof5%sodiumtaurocholateintothecholedochus.Results　OctreotidecoulddecreaseWet/drylu

4、ngratiosignificantlyatallindicatedtime-point.At12thhafterinductionofANP,PaO2,proteincontentinBALF,plasmaendotoxinlevel,serumNO2-/NO2-,andNOSactivityweremuchlowerinoctreotide-treatedratsthanthoseinuntreatedgroup(P＜0.05).LeukocytecountinBALFwasalsodecreasedwithsomeextentbutwitho

5、utstatisticalsignificant(P＞0.05).SerumconcentrationofTNFαwaslowerdramaticallyinoctreotide-treatedgroupthaninANP+NSgroup.Pathologicalstudiesshowedthatinflammatorycellinfiltrationandpulmonaryinterstitialedemawereimproved,andtypeIIepithelialcellswerewellpreservedinoctreotide-trea

6、tedgroup.Conclusion　OctreotideshoweditsbeneficialeffectsonlunginjuryinbothfunctionalandmorphologicalterationsduringthecourseofANP.Regulatoryrolesofoctreotideonpro-inflammatoryfactors,suchasendotoxin,TNFα,andNOcouldbeconsideredasoneofthemajormechanismsunderthissituation.　　【Keyw

7、ords】　Acutepancreatitis；　Somatostatin；　Acuterespiratorydistresssyndrome；　Cytokine　　我们以牛磺胆酸钠诱导大鼠，观察奥曲肽对急性坏死性胰腺炎(acutenecroticpancreatitis,ANP)大鼠肺脏形态和功能变化的影响以及对炎症介质的调节作用，现报道如下。　　材料和方法　　1.实验动物与主要试剂：雄性Spraque-Dawley大鼠63只，体重300～350g。TNFα测试盒为Endogen公司产品。　　2.方法：(1)ANP模型制备：大鼠实验前禁食12h，

8、自由饮水。ANP模型制备参照文献［1］。假手术组(shamoperation,SO)大鼠仅作剖腹术。(2)实验分组：大鼠随