胃癌组织中基质金属蛋白酶-9和整合素β3 mRNA表达的临床意义.doc

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1、胃癌组织中基质金属蛋白酶-9和整合素β3mRNA表达的临床意义作者：杨月明叶再元赵仲生王元宇【摘要】目的研究基质金属蛋白酶-9（MMP-9）和整合素β3（Integrinβ3）mRNA在胃癌组织中的表达及与临床病理参数、肿瘤微血管密度、生存期的关系。方法应用原位杂交和免疫组化，检测105例胃癌组织中MMP-9、Integrinβ3mRNA和CD34的表达。结果MMP-9和Integrinβ3mRNA阳性表达率分别为58.10％和60.95％，其阳性表达与胃癌生长方式、浸润深度、脉管侵犯、淋巴结及远处转移均密切相

2、关（P均＜0.01）；MMP-9、Integrinβ3mRNA的阳性表达病例的平均血管数均高于阴性表达病例的平均血管数（P均＜0.05）；MVD值分别与MMP-9和Integrinβ3mRNA的表达水平呈正相关（P均＜0.05）；MMP-9和Integrinβ3mRNA的阳性表达以及MVD值≥39.5病例的5年生存率显著低于阴性表达和MVD值＜39.5的病例；MMP-9和Integrinβ3mRNA表达存在显著相关性（P＜0.05）。结论MMP-9、Integrinβ3可促进胃癌血管生成，并参与肿瘤浸润转移过程

3、，可以作为反映胃癌生物学行为和判断预后的有效指标。【关键词】胃癌基质金属蛋白酶-9整合素β3转移预后　　【Abstract】ObjectiveToinvestigatetheexpressionofmatrixmetalloproteinase-9(MMP-9)andintegrinbeta-3mRNAingastriccarcinomaandtofindthecorrelationbetweentheexpressionrateofthemandclinicopathologicindex，microvess

4、eldensityandsurvivaltimeofgastriccarcinomapatients.MethodsInsituatehybridizationandimmunohistochemicaltechniqueswereusedtodetectthegeneexpressionofMMP-9,Integrinbeta-3andCD34in105gastriccarcinomaspecimens.ResultsInsituhybridizationrevealedthatpositiveexpress

5、ionrateofMMP-9andIntegrinbeta-3mRNAwas58.10％and60.95％，respectively.Therewasasignificantcorrelationbetweentheexpressionofthemandgrowthpattern,depthoftumorinvasion，vesselinvasion,lymphnodeanddistantmetastasis(P＜0.01).MeantumorMVDofpatientswithpositivemRNAexpre

6、ssionwassignificantlyhigherthanthosewithnegativemRNAexpression(P＜0.05).Inaddition,MVDvaluewaspositivelycorrelatedwiththeexpressionlevelofMMP-9andIntegrinbeta-3mRNA(P＜0.05).ThefiveyearsurvivalrateofpatientswithpositivemRNAexpressionandmeanMVDvalue≥39.5/mm2wer

7、esignificantlylowerthanthoseofpatientswithnegativemRNAexpressionandmeanMVDvalue＜39.5/mm2;TheexpressionofMMP-9wassignificantlycorelatedwiththeexpressionofintegrinβ3(P＜0.05).ConclusionTheexpressionofMMP-9andIntegrinβ3canpromotetumorangiogenesisandcontributes5t

8、otumorinvasionandmetastasisingastriccarcinoma.Theexpressionofthemmayserveasvaluableindicatorsofbiologicalbehaviourandprognosisofgastriccarcinoma.　　【Keyword】GastriccarcinomaMatrixmetalloproteinas