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1、胃癌组织中runx3mRNA和蛋白的表达及其临床意义论文李华,李勇,范立侨,王力利,赵群,赵雪峰,焦志凯【关键词】,胃肿瘤;runx3基因;印迹法,蛋白质;逆转录聚合酶链反应Expressionofrunx3mRNAandproteiningastriccarcinomaanditscorrelationeters【Abstract】AIM:Toinvestigatetheexpressionofrunx3genemRNAandproteininhumangastriccarcinomaandsurroundingnormalti
2、ssueanditscorrelationeters.METHODS:RNAandprotein60humangastriccarcinomaandsurroundingnormaltissuespecimens.Reversetranscriptionpolymerasechainreaction(RTPCR)and:Marker;N:正常组织;T:肿瘤.图1RTPCR分析runx3mRNA在胃癌组织和正常粘膜内的表达(略)2.2runx3蛋白在胃癌和正常粘膜组织中的表达runx3A值在胃癌组织和正常粘膜组织中分别为(10828
3、±989)和(47833±7566)(图2),两组相比有统计学意义(t=35.57,P0.0001).N:正常胃粘膜组织;T:肿瘤组织.图2odPatho1,2000,13(6);627-631.[5]Fangappingofaminimaldeletedregioninhumanhepatocellularcarcinomato1p36.13p36.23andmutationalanalysisoftheRIZ(PRDM2)genelocalizedtotheregion[J].GenesChromosomesCancer,20
4、00,28(3):269-275.[6]ItoY.MolecularbasisoftissuespecificgeneexpressionmediatedbytheruntdomaintranscriptionfactorPERP2/CBF[J].GenesCells,1999,4(12);685-696.[7]L1andCBFbeta[J].EMBOJ,2000,19(12);3004-3015.[8]LiQL,ItoK,SakakuraC,etal.CausalrelationshipbeturaG,SatoM,etal.Pr
5、omotermethylationstatusofDAPkinaseandRUNX3genesinneoplasticandnonneoplasticgastricepithelia[J].CancerSci,2003,94(4):360-364.[10]MiyazonoK,SuzukiH,ImamuraT,etal.RegulationofTGFbetasignalinganditsrolesinprogressionoftumors[J].CancerSci,2003,94(3):230-234.[11]KimTY,LeeHJ
6、,Hancancersandpremalignantstagesofgastriccarcinoma[J].LabInvest,2004,84(4):479-484.[12]LevanonD,BrennerO,OttoF,etal.Runx3knockoutsandstomachcancer[J].EMBORep,2003,4(6):560-564.