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1、吗啡对垂体后叶素致兔心肌损伤的影响【摘要】目的观察吗啡预处理垂体后叶素引起的兔心肌损伤后血压、心电图和血清心肌肌钙蛋白I(cTn-I)的含量变化,并观察心肌病理切片,探讨吗啡对受损心肌预处理的影响。方法将成年中国白兔24只随机分为三组,对照假手术组(A组),手术模型完成稳定45min后予0.9%生理盐水0.2~0.3mL/kg,30s静脉注射完毕;药物损伤心肌组(B组),45min后予垂体后叶素(2.5U/kg)0.2~0.3mL/kg,30s注射完毕;吗啡预处理组(C组),予吗啡0.65mg/kg静脉推注,45min后予垂
2、体后叶素(2.5U/kg)0.2~0.3mL/kg,30s注射完毕,记录给药前后平均动脉压(MAP)、中心静脉压(CVP)、HR及T波变化;测定血清cTn-I含量;观察给药48h后白兔心肌组织的变化。结果与A组比较,B组MAP、CVP、T波明显升高(P<0.01),HR明显下降(P<0.01);与B组相比,C组MAP、CVP、T波明显下降(P<0.01),T波B、C组明显高于A组(P<0.01)。血清cTn-I水平A、C组低于B组(P<0.01)。结论吗啡预处理对垂体后叶素所致的兔心肌损伤有保护作用。【关键词】心肌损伤;吗啡
3、;肌钙蛋白I;双抗夹心免疫吸附法;兔 Abstract:ObjectiveToobservethechangeofbloodpressure,electrocardipgramandTroponinIaftermorphinepretreatmentonacutemyoardialinfarctioninducedbypituitrininrabbits,andtocomparethemwiththechangeofpathostaining,andtoexploretheeffectofmorphinepretreatm
4、entonacutemyocardialischemicinjury.MethodsTwenty-fourrabbitsweredividedrandomlyintothreegroups:controlgroup(GroupA),pituitringroup(GroupB)andmorphinepretreatmentgroup(GroupC).TheinformationincludedMAP、CVP、HRandTwavewereobservedandrecordedatdifferenttimepoints.Itwas
5、dividedintotwoobservingphrasesof24h,48handdeterminedthecontentsofbloodserumcTn-Iandcaridiacpathostainof48h.Results(1)Changesinmyocardiacmechanics:myocardiacmechanicshadadynamicchangeafterinjectionofpituitrin2.5U/kgweight.parametersbetweenandwithingroupsthroughoutth
6、eexperimentalperiodexceptischemiagroup,whichhadasignificantlreductioninHRandasignificantincreaseinMAP,CVPandTwave.Comaredwithothergroups.GroupCcomparedwithGroupA,MAPandCVPateachtimepointtherewasnosignificantstatisticaldifference,HR(1min)decreased(P<0.05),Twaveincre
7、ased(P<0.01).ComparedBgroupwithgroupA,MAP(1min)significantlyincreased(P<0.01),CVPsignificantlyincreasedfrom15secto5min(P<0.01),HRsignificantlyloweredin15secto10min(P<0.01),Twavesignificantlyincreased(P<0.01).ComparedGroupBwithCgroup,HRsignificantlydecreasedatthepoi
8、ntoffrom30secto2min(P<0.01),MAP,CVPandTwavewasnosignificantstatisticaldifferenceatdiffernettimepoints.(2)BloodserumcTn-Ishowedsignificantdifferen