糖尿病大鼠膀胱收缩功能改变与M3受体关系的实验研究.doc

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1、糖尿病大鼠膀胱收缩功能改变与M3受体关系的实验研究【摘要】目的探讨糖尿病性膀胱发病机制中逼尿肌M3受体的改变情况。方法以链脲佐菌素诱导2型糖尿病大鼠成模。应用尿动力仪检测逼尿肌收缩功能；Westernblot、RT-PCR方法检测膀胱M3受体含量。结果病程2周时糖尿病组大鼠膀胱逼尿肌收缩力增加伴M3受体表达增强；病程4周时糖尿病组大鼠膀胱逼尿肌收缩力低于正常对照组，但尚无统计学意义；病程12周时糖尿病组大鼠膀胱逼尿肌收缩力降低伴M3受体表达减少。结论膀胱收缩功能和M3受体蛋白含量及M3受体mRNA的含

2、量三者呈同向变化关系。在糖尿病大鼠发病早期三者均升高，但是随着病程进展却都降低了。这可能是早期糖尿病性膀胱病变的发病机制之一。　　【关键词】糖尿病性膀胱；逼尿肌；收缩功能；M3受体　　AlterationofdetrusorcontractionandM3subtypemuscarinicreceptorsinthetype2diabeticraturinarybladder　　【Abstract】ObjectiveToresearchintothealterationofdetrusorcontra

3、ctionandM3subtypemuscarinicreceptorsinthediabeticcystopathy(DCP).MethodsThetype2diabeticratswereinducedbytheintraperitonealinjectionofStreptozotocin(STZ)(90mg/kg)andhighcaloricdiet.Theidentical-week-oldWistarratswereutilizedascontrolrats.Westudiedthecon

4、tractilefunctionsofwholebladder.FordeterminationofM3muscarinicreceptormRNAinthebladdertissue,themethodofRT-PCRwasemployed.TheamountofM3receptorproteinintheratbladderbodytissuewasmeasuredbythemethodofWesternblot.ResultsTheresultsofthewholebladdercontract

5、ileforceshowedthatthecontractilefunctionofthe2-weekdiabeticratsbladderwasincreased.Thecontractilefunctionofthe4-weekdiabeticratsbladderwasdecreasedwhencomparedtothecontrolrats.When1mmol/LCarbacholwasexisted,thecontractilefunctionofthe4-weekdiabeticratsb

6、ladderwasdecreased,buthavenostatisticalsignificance.Thecontractilefunctionofthe12-weekdiabeticratsbladderwasdecreased（P<0.01，n＝8）.M3muscarinicreceptormRNAinthe2-weekdiabeticratsbladderwasincreased.TheamountofM3muscarinicreceptormRNAinthe4-weekdiabeti

7、cratsbladderwassignificantlydecreased.TheamountofM3muscarinicreceptormRNAinthe12-weekdiabeticratsbladderwassignificantlydecreased.WesternblotdemonstratedthattheamountofM3muscarinicreceptorproteininthe2-weekdiabeticratsbladderwasdecreased.ConclusionThese

8、resultssuggestthatthealteratetrendofdetrusorcontraction,M3muscarinicreceptormRNAandM3subtypemuscarinicreceptorsproteininthetype62diabeticraturinarybladderweresimilar.Attheearlystageofdiabetesmellitus,However,asdiabetesmellitusisd