重组人促红细胞生成素对脓毒症大鼠肺脏的保护作用[权威资料]

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1、重组人促红细胞生成素对脓毒症大鼠肺脏的保护作用本文档格式为WORD,若不是word文档,则说明不是原文档。最新最全的学术论文期刊文献年终总结年终报告工作总结个人总结述职报告实习报告单位总结　　【摘要】目的探讨重组人促红细胞生成素（recombinanthumanerythropoietin，rHuEPO）对内毒素所致大鼠脓毒症急性肺损伤的保护作用。方法45只雄性SD大鼠随机（随机数字法）分为对照组、模型组、rHuEPO组，每组均15只。经静脉推注脂多糖LPS（6mg/kg）复制急性肺损伤（ALI）的动物模型，rHuEPO组造模前60min静脉推注rHuEPO（5000U/kg），观

2、察12h后处死。留取颈动脉血及肺组织标本。测定氧和指数（OI）、动脉血氧分压（PaO显著降低，差异具有统计学意义（P、IL-6、iNOS水平进而调节炎症反应，对脓毒症所致急性肺损伤具有一定的保护作用。　　【关键词】重组人促红细胞生成素；脂多糖；脓毒症；急性肺损伤　　ProtectiveeffectsofrecombinanthumanerythropoietinonacutelunginjuryinducedbysepsisinratsSANGZhen-zhen，XUYun，SHENGYing-jie，ZHAOMin.DepartmentofEmergencyMedicine，Sh

3、enjingHospitalofChinaMedicalUniversity，Shenyang110004，China　　Correspondingauthor：ZHAOMin，Email：zhaom@sj-hospital.org　　【Abstract】ObjectiveToinvestigatetheprotectiveeffectsofrecombinanthumanerythropoietin（rHuEPO）onacutelunginjury（ALI）inducedbylipopolysaccharide（LPS）.MethodsFourty-fiveratswereran

4、domly（randomnumber）assignedtothreegroups，namelycontrolgroup，modelgroup，andrHuEPOgroup.ALIwasinducedbyintravenousinjectionofLPS（6mg/kg）.TherHuEPO（5000U/kg）wasinjectedintravenouslyintorats60minbeforeLPSchallenge.Thegeneralstatusofratswasobserved.Twelvehoursaftermodeling，theratsweresacrificedandt

5、hetissuesamplesincludinglungtissueandbloodwerecollected.PaO）］，andinduciblenitricoxidesynthase（iNOS）weredetected.CytokineswereassayedwithELISAmethod.Pathologicalchangesoflungtissueswereobservedunderlightmicroscopeandtransmissionelectronmicroscopy.Results（1）Comparedwiththecontrolgroup，PaOweresig

6、nificantlylower（P，IL-6andiNOSinserumofratsinthecontrolgroupwerelowerthanthoseinthemodelgroupandtherHuEPOgroup（P，IL-6andiNOSofratsintherHuEPOgroupweresignificantlylowercomparedwiththemodelgroup（P<0.01）.（4）Thelightmicroscopyandthetransmissionelectronmicroscopyshowedthemodelgrouphadhistopathologi

7、cchangeswithacutediffuselunginjurymanifestedbyintra-alveolarhemorrhage，exudate，inflammatorycellsinfiltration，ⅠtypeandⅡtypeepithelialcellnecrosisanddetachment，andthepathologicalchangesoflungtissueintherHuEPOgroupwerenotasseriousasthosein