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1、植物甾醇酯酶促合成及其干预脂代谢功能研究//.paper.edu.cn-1-中国科技论文在线Lipase-catalyzedsynthesisandthelipidmetaboliceffectofphytosterolesters#PangMin,ZhengHaijie,JiangShaotong**(SchoolofBiotechnologyandFoodEngineering,HefeiUniversityofTechnology,Hefei,230009,5P.R.China)Foundations:SpecializedResea
2、rchFundfortheDoctoralProgramofHigherEducation(No.20110111120021)Briefauthorintroduction:PangMin,PhD,AssociateProfessor.ThemaindirectionofresearchisinlipidnutrientsCorrespondanceauthor:JiangShaotong,professer.Themaindirectionisintheresearchoffoodscience.Abstract:Inthispaper
3、,theesterificationsofphytosterolwitholeicacidcatalyzedbyCandidarugosalipaseinorganicsolventwerestudied.Theesterificationconditions(temperature,substratemolarratio,reactiontimeandcatalystamount)wereoptimizedbyresponsesurfacemethodology(RSM).Thephytoserollinoleicacidesterwas
4、alsopreparedvialipase-catalyzed.Thelipidmetaboliceffectof10equivalentintakesofphytoserololeic/linoleicacidestersandthatofdifferentintakesofthesameesterwerecompared.Theresultsdemonstratedthatthehighestphytoserololeicacidesteresterificationdegreeof77.43%wasobtainedundertheop
5、timizedconditions:temperatureof41℃,reactiontimeof19h,substratemolarratio(oleicacid:phytosterol)of2.4:1andcatalystamountof7%.ThechemicalstructureofphytoserololeicacidesterwasconfirmedbyFT-IRandGC-MS.Thepreparedphytosterol15estersdecreasedsignificantlyTC,LDL-C,LDL-C/HDL-C,Ap
6、oAⅠ,ApoAⅠ/ApoBinserumandliverweight,HI,hepaticTC,hepaticTG,whiletheyalsoincreasedsignificantlyHDL-CandApoBinserum.NosignificantchangeswerefoundintheconcentrationsofTG.Incontrast,linoleicacidestercouldhaveaadvantageeffectoncholesterol-loweringandantioxidantcapacityoveroleic
7、acidester.Keywords:Lipidnutrient;Lipase-catalyzed;Phytosterolesters;Cholesterol-lowering;Antioxidant20capacity0IntroductionDyslipidemia,includingelevationoftotalcholesterol(TC),lowdensitylipoproteincholesterol(LDL-C)andtriacylglycerol(TG)concentrationsandadecreaseinhighden
8、sitylipoproteincholesterol(HDL-C)concentrationintheblood,isthemajorriskfactorofatheroscle
