心肌梗死患者外周血单个核细胞基质金属蛋白酶9mrna的表达

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1、心肌梗死患者外周血单个核细胞基质金属蛋白酶9mRNA的表达【摘要】目的：探讨单个核细胞(PBMC)中基质金属蛋白酶9(MMP9)在心肌梗死(AMI)病理生理机制中的作用。方法:用电化学发光法检测30例AMI患者与对照组30例血浆肌钙蛋白T(cTnT)的含量;用ELISA法测定血浆MMP9的含量及RTPCR检测单个核细胞中MMP9mRNA的表达水平。结果:AMI患者在6h及1周内MMP9的含量分别为(697±143)ng/mL,(597±102)ng/mL较对照组的(342±165)ng/mL显著增高(P<0.05)

2、，与cTnT的变化差异无统计学意义(P>0.05)，且呈正相关(r=0.82,P<0.05)。AMI患者单个核细胞MMP9mRNA表达显著增强。结论:AMI患者PBMC处于炎性状态，MMP9mRNA表达增强，并可能由此导致血浆MMP9水平增高。监测血浆MMP9的水平有可能成为了解AMI发生发展的一个重要诊断指标，为AMI的治疗和预后提供临床依据。【关键词】心肌梗死;基质金属蛋白酶9;单个核细胞;肌钙蛋白TMatrixmetalloproteinase9mRNAexpressionofperipheralblo

3、odmononuclearcellinpatientswithacutemyocardialinfarctionRONGHong1,SANGShenggang2(1.DepartmentofStomatology;2.ClinicalLaboraryDepartment,Affiliated11HospitalofHainanMedicalCollege,Haikou570102,China)[ABSTRACT]Objective:Toinvestigatetheroleofmatrixmetalloproteinase9(M

4、MP9)inthedevelopmentofacutemyocardialinfarction(AMI).Methods:TheplasmatroponinTofboth30acutemyocardialinfarctionsand30controlcasesweremeasuredbyautomaticelectrochemiluminescenceanalysis;plasmaMMP9levelswerequantifiedwithenzymeimmunoassaymethod(ELISA);andmRNAexpressi

5、onofMMP9extractedfrommononuclearcellweredetectedbyRTPCRmethod.Results:MMP9levelsoftheAMIgroupwithin6handduringthefirstweekwere(697±143)ng/mLand(597±102)ng/mLseparately,bothofwhichweresignificanthigherthan(342±165)ng/mLofthecontrolgroup(P<0.05).Therewasapositivec

6、orrelationbetweenthelevelsofMMP9andcTnT(r=0.82,P<0.05)thoughnosignificantdifferencewasfound.TheMMP9mRNAexpressionswereobviouslyamplifiedinperipheralbloodmononuclearcells.Conclusion:ThestudydemonstratsthattheconcentrationandexpressionofMMP9mRNAaresignificanthighe

7、rinpatientswithmyocardialinfarctionthanthatincontrolgroup.TheactivatedperipheralbloodmononuclearcellsandenhancedMMP9mRNAexpressionmayresultintheincreaseoftheMMP9concentrationinplasmaofthepatient.Thereby,theMMP119levelofplasmacanbevaluedasanimportantindexforthediagn

8、osis,treatmentandprognosisofacutemyocardialinfarction.　　[KEYWORDS]Matrixmetalloproteinase9;Acutemyocardialinfarction;Mononu