川芎嗪对兔肾缺血再灌注损伤超微结构改变的干预作用

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1、川芎嗪对兔肾缺血再灌注损伤超微结构改变的干预作用【摘要】目的:观察兔肾缺血再灌注损伤超微结构改变，探讨川芎嗪注射液的干预作用。方法:复制家兔肾缺血再灌注损伤模型，日本大耳白兔30只，随机均分为三组：假手术组（S），缺血再灌注组（IR）和缺血再灌注+川芎嗪注射液组（LZ）。使用透射电子显微镜观察各组肾组织标本超微结构的变化，作对比分析。结果:IR组主要异常改变有：①肾小球毛细血管腔狭窄，中性粒细胞附壁，大部分内皮细胞核固缩、染色体边集，细胞质内空泡形成，线粒体空泡变性。②多数脏层上皮细胞轻度核固缩，个别线粒

2、体空泡变性，足突相互黏连，可见局灶性融合现象和微绒毛化现象。③近曲小管上皮细胞核固缩，胞质内出现较多空泡，溶酶体和致密颗粒沉积增多，线粒体数量减少，部分线粒体嵴断裂、空泡化，腔面微绒毛稀疏并有轻度肿胀现象。LZ组大部分标本的上述部位存在不同程度损伤性改变，但比IR组均明显减轻，其中近曲小管上皮细胞超微结构的改善较显著。结论:川芎嗪注射液可减轻肾缺血再灌注损伤所致的肾小球和肾小管超微结构的异常变化。【关键词】肾脏缺血再灌注损伤超微结构川芎嗪注射液Abstract:Objective:Toobservethe

3、ultrastructuralchangesinrenaltissueduringrenalischemiareperfusioninjury9inrabbitsandstudytheinterferingeffectsofligustrazineinjection.Methods:Themodelofrenalischemiareperfusioninjuryofrabbitwasestablishedinvivo.Thirtyrabbitsweredividedintothreegroupsrando

4、mly:shamoperationgroup(Sgroup),ischemiareperfusioninjurygroup(IRgroup)andischemiareperfusioninjuryplusligustrazineinjectiongroup(LZgroup).Theultrastructuralchangesinrenaltissuewereobservedunderelectronmicroscopein5hoursafterreperfusion.Results:Theabnormal

5、ultrastructureinIRgroupincludedthat①Thecavitiesoftheglomerularcapillarynarrowed,polymorphonuclearleucocytesadheredtothewalloftheveessel,mostoftheendotheliashowednucleuspyknosisandstainingthick,chromosomegatheredneartonuclearmembrance,vacuolizationincytopl

6、asmandsomemitochondiavacuolardegenerationwereseen.②Lightdegreenucleuspyknosisdemonstratedinmostoftheviscerallayeroftheepithelia,individualmitochondrialvacuolardegeneration,footprocessmutualadhesionwereseenwithfocalcoalescenceandmicrovillus.③epithelialcell

7、sofproximalconvolutedtubuleappearednucleuspyknosis,morevacuolizationwasseenincytoplasm,lysosomeanddensegranuleincytoplasmincreased,numberofmitochondrialvacuolizationdecreased,itscrestpartly9fragmented,microvillirarefactionandswellingwerefound.Theultrastru

8、cturalinjuriesoftherenaltissuesinLZgroupwereobviouslyalleviatedascomparedtoIRgroupandimprovementofultrastructureinepithelialcellsofproximaltubulewasnotable.Conclusion:Ligustrazineinjectioncanmitigatetheabnormalchang