冬虫夏草对糖尿病大鼠纤溶系统的影响

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1、冬虫夏草对糖尿病大鼠纤溶系统的影响作者：陈叶傅余芹方华伟韩亚莉杜月娟【摘要】　　目的观察冬虫夏草(CS)对糖尿病肾病(DN)大鼠纤溶系统功能的影响。方法44只大鼠随机分为正常对照组、模型组(给予链脲佐菌素55mg/kg体重腹腔注射)，CS早期干预组及冬虫夏草晚期干预组，12w后测体重、肾重、血糖、血肌酐(Scr)、24h尿蛋白等指标，光镜观察肾脏病理变化，免疫组化法分别检测组织型纤溶酶原激活物(tPA)/纤溶酶原激活物抑制剂表达(PAI)。结果模型组肾重、血糖、Scr、24h尿蛋白均明显高于正常对照组(P＜0.01

2、)，PAI表达明显高于正常对照组，体重、tPA表达明显低于正常对照组；两干预组肾重、Scr、血尿素氮(BUN)、24h尿蛋白、tPA表达均高于模型组(P＜0.05)，低于正常对照组(P＜0.01)，体重、PAI表达低于模型组，高于正常组，血糖较模型组无明显变化；冬虫夏草早期干预组效果优于晚期干预组(P＜0.05)。结论DN时存在tPA/PAI失衡，冬虫夏草治疗DN的机制之一为矫正tPA/PAI失衡。【关键词】冬虫夏草；糖尿病肾病；纤溶酶原激活剂；纤溶酶原激活剂抑制剂【Abstract】ObjectiveToi

3、nvestigatetheeffectsofcordycepssinensis(CS)onfibrinolyticsystemofdiabeticrats.11Methods44ratsweredividedinto4groups:control(n=10),model(n=12,intraperitonealinjectionwith55mg/kgSTZ),earlyandlateCStreatedgroups(n=11).1ratwasfailedtomademodel,1ratdiedinmodelgroupa

4、nd1ratdiedinearlyCStreatedgroup.12weekslater,bodyweight,renalweight,serumglucose,serumcreatinine,24hoururinaryproteinexcretionwereexamined.Therenalpathologicalchangeswereobservedbylightmicroscopeandthelocalexpressionsofrenalplasminogenactivatorinhibitor1(PAI

5、1)andtissuetypeplasminogenactivator(tPA)weremeasuredbyimmunohistochenmicaltechnique.ResultsInmodelgroup,renalweight,serumglucose,serumcreatinine,24hoururinaryproteinexcretionandPAIwereincreasedsignificantly(P＜0.01),whilebodyweightandtPAweredecreasedcompared

6、withcontrolgroup(P＜0.01).Therenalweight,serumcreatinine,24hoururinaryproteinexcretion,tPAwereincreasedinearlyandlateCStreatedgroupscomparedwiththoseofmodelgroup(P＜0.05),andtheaboveindexesweredecreasedcomparedwiththoseofcontrolgroup(P＜0.01).Bodyweight,PAIwered

7、ecreasedinearlyandlatetreatedgroupscomparedwiththoseofmodelgroupwhilewereincreasedcomparedwiththoseofcontrolgroup(P＜0.05).Serumglucoseininearlyandlatetreatedgroupshadnoobviousdifferencecomparedwith11modelgroup.TheeffectinearlyCStreatedgroupwasbetterthanthatofla

8、teCStreatedgroup(P＜0.05).ConclusionsThereisdisbalanceindiabeticnephropathyandtherectificationofthetPA/PAIdisbalanceisoneofmechanismsofCStreatingdiabeticnephropathy.【Keyword